中国生态农业学报2025，Vol.33Issue(6)：1094-1102,9.DOI:10.12357/cjea.20240540
不同生态型蚯蚓对有机茶园土壤真菌群落结构及多样性的影响
Effects of different ecological type earthworms on the structure and diversity of soil fungal community in an organic tea plantation
摘要
Abstract
Aging tea trees,degradation of soil fertility in tea gardens,and poor soil biodiversity have become important factors that limit the yield and quality of organic tea.Earthworms play an important role in the regulation of soil ecosystem services.The inocula-tion of two widely reared earthworm pioneer species was tested using field experiments on a tea plantation in China.Field experi-ments were conducted to explore the effects of inoculation with different types of earthworm on soil fungal communities in organic tea gardens.Four treatments were set up,including:Eisenia fetida inoculation with a inoculation density of 300 individual·m-2(E),Metaphire guillelmi inoculation with a inoculation density of 100 individual·m-2(P),simultaneous inoculation of E.fetida with a in-oculation density of 150 individual·m-2 and M.guillelmi inoculation with a inoculation density of 50 individual·m-2(EP),and control(CK).Soil fungal communities were investigated using high-throughput sequencing.The results showed that the community structure and functional groups of the soil fungi changed significantly.Compared with CK treatment,inoculation with earthworms signific-antly increased the number of operational taxonomic unit(OUT)in the soil fungi,and the number of dominant OTU increased from 100 to 269.Compared with CK treatment,inoculating earthworms increased the α diversity of soil fungal community;Shannon,Simpson,and Fisher indices under the treatments with single type earthworm inoculation also increased(P＜0.05).Ascomycota and Basidiomycota were the dominant fungal phyla in tea garden soil.Compared with CK treatment,Ascomycota further increased under the treatments with single type earthworm inoculation(E and P treatments)and decreased slightly under EP treatment.Compared with CK treatment,increase in relative abundance of Mortierellomycota under EP treatment(10.16％)was the largest.EP treatment showed the best population uniformity,and more undefined fungi appeared with a proportion of 29.61％.Xylariales decomposes lit-ter,thus the EP treatment exhibited a stronger ability to decompose dead branches and leaves than single type earthworm inoculation treatment,which is more beneficial for increasing soil nutrients in organic tea gardens.Compared with conventional organic tea gar-dens,inoculation with earthworms reduced the relative abundance of saprotrophic fungi while improving the growth of pathotroph-saprotroph-symbiotroph fungi.In addition,EP treatment promoted the growth of saprotroph-symbiotroph fungi.Generally,in biolo-gically and physically degraded soil systems,mutual promotion between earthworm species outweighs competition.Mixed inocula-tion of different types of earthworms affected pathogen control,which can help control potential pathogens,thereby providing a sus-tainable solution for improving soil biodiversity and enhancing soil quality in organic tea gardens.Inoculating earthworms is an ef-fective and feasible ecological measure for improving soil quality in organic tea gardens.关键词
蚯蚓/真菌群落多样性/有机茶园/真菌群落结构Key words
earthworm/fungal community diversity/organic tea garden/fungal community structure分类
农业科技引用本文复制引用
张香凝,陈涛,史福刚,李太魁,赵丰华,郭战玲..不同生态型蚯蚓对有机茶园土壤真菌群落结构及多样性的影响[J].中国生态农业学报,2025,33(6):1094-1102,9.基金项目
国家现代农业产业体系建设专项(CARS-23)、河南省科技研发联合基金重大项目(235101610015)、河南省优势特色农业产业科技支撑性行动计划(20230701003,20220701003)和河南省农业科学院自主创新项目(2024CZ050)资助 This study was supported by the Construction of National Modern Agricultural Industry System(CARS-23),Henan Province Science and Technology Research and Development Joint Fund(235101610015),the Characteristic Agriculture Technology Support Program in Henan(20230701003,20220701003)and the Independent Innovation Project in Henan Academy of Agricultural Sciences(2024CZ050). （CARS-23）
