饲料研究2025，Vol.48Issue(9)：111-117,7.DOI:10.13557/j.cnki.issn1002-2813.2025.09.019
微生物对银杏叶中银杏酚酸降解效果探究
Study on degradation effect of ginkgolic acid in Ginkgo biloba leaves by microorganisms
摘要
Abstract
The study aimed to screen the dominant ginkgolic acid degrading strains and optimize their degradation conditions.The ultrasonic assisted n-hexane solvent extraction method was used to extract ginkgolic acid from Ginkgo biloba leaves.The high performance liquid chromatography detection method was established by optimizing the mobile phase ratio,flow rate,temperature and other chromatographic parameters.The results showed that the optimized optimal chromatographic conditions were acetonitrile:0.1％phosphoric acid of 90∶10,column temperature of 30℃,flow rate of 1.0 mL/min,and detection wavelength of 310 nm.Compared with Bacillus subtilis,yeast,Trametes hirsuta,etc.,Trichophyton had the best degradation ability for ginkgo phenolic acid.The degradation conditions of Trametes hirsuta were optimized by the single-factor and response surface methods as follows:Fermentation temperature of 24 ℃,fermentation for 5 days,inoculation amount of 10％,liquid loading volume of 12 mL,and rotational speed of 225 r/min.Under these conditions,the average degradation rate of ginkgolic acid could reach 77.67％.The study indicates that Trametes hirsuta has a good degradation ability for ginkgo phenolic acid.关键词
银杏叶/银杏酚酸/超声波提取/毛栓菌/高效液相色谱Key words
Ginkgo biloba leaves/ginkgolic acid/ultrasonic extraction/Trametes hirsuta/high performance liquid chromatograph分类
农业科技引用本文复制引用
王君,王佳宁,王若楠,龚洪伶,朱亚男,胡高爽..微生物对银杏叶中银杏酚酸降解效果探究[J].饲料研究,2025,48(9):111-117,7.基金项目
河北省现代农业产业技术体系建设专项基金(项目编号:HBCT2024270206) （项目编号:HBCT2024270206）
沧州市重点研发计划项目(项目编号:204204004) （项目编号:204204004）
沧州师范学院现代微生物发酵与细胞工程创新团队(项目编号:cxtdl2303) （项目编号:cxtdl2303）
