生物技术通报2025，Vol.41Issue(6)：1-11,11.DOI:10.13560/j.cnki.biotech.bull.1985.2024-1160
CRISPR/Cas12a基因编辑技术在植物中的研究进展
Current Progress and Applications of CRISPR/Cas12a Gene Editing Technology in Plants
摘要
Abstract
The CRISPR/Cas system provides bacteria and archaea with adaptive immunity against viruses and plasmids by using crRNAs to guide the silencing of invading nucleic acids.The development and optimization of CRISPR/Cas system have provided a variety of gene editing tools for life science research,such as CRISPR/Cas9 genome editing technology,CRISPR/Cas12a genome editing technology,base editing,and prime editing.These tools are capable of precisely editing target genomes to generate various types of desired mutants,and show great application potential in functional genomics,construction of disease models,gene therapy and crop breeding.CRISPR/Cas12a genome editing technology is developed based on Class II type V CRISPR.Cas12a(Cpf1)is a single RNA-guided endonuclease lacking tracrRNA.Besides,it presents distinct characteristics,such as the ability to utilize TTTV PAM,easy to engineer,and multiplex genome editing.Due to these advantages,CRISPR/Cas12a has been successfully applied in diverse species including animals and plants since the first report.Therefore,CRISPR/Cas12a will have a high prospect of providing important technical support for gene therapy and crop breeding.In this review,we provide a detailed introduction to the CRISPR/Cas12a genome editing technology,as well as the precise genome editing technologies developed based on CRISPR/Cas12a.We also focus on the strategies for optimizing CRISPR/Cas12a through improving editing efficiency,expanding the targeting scope,and enhancing specificity.Finally,we summarize the current applications of CRISPR/Cas12a in the creation of new plant germplasm.This review is aimed to facilitate the further application of CRISPR/Cas12a in crop improvement.关键词
CRISPR/Cas12a/基因组编辑/优化/遗传改良Key words
CRISPR/Cas12a/genome editing/optimization/genetic improvement引用本文复制引用
霍贯中,张欣濡,田士军,李君..CRISPR/Cas12a基因编辑技术在植物中的研究进展[J].生物技术通报,2025,41(6):1-11,11.基金项目
国家自然科学基金青年基金项目(32402564),河北省自然科学基金项目(C2023204119) （32402564）
