生物技术通报2025，Vol.41Issue(6)：99-108,10.DOI:10.13560/j.cnki.biotech.bull.1985.2024-1136
大豆抗豆卷叶螟GmKTI1-like的功能研究
Functioal Analysis on GmKTI1-like Gene of Soybean Resistance to Bean Pyralid(Lamprosema indicata)
摘要
Abstract
[Objective]Kunitz-type trypsin inhibitors play an important role in enhancing plant resistance to pests.Prior research conducted by our group has demonstrated a significant association between the Kunitz-type trypsin inhibitor GmKTI1-like and resistance to the soybean leaf borer.This study is aimed to investigate the function of the GmKTI1-like gene and investigate its role to provide key resistance genes and new breeding materials for the cultivation of insect-resistant soybean varieties.[Method]GmKTI1-like was cloned from soybean leaves.Then bioinformatics methods were used to analyze their physicochemical properties,protein structure,physical positioning of chromosomes and subcellular localization,and RT-qPCR was used to analyze the expression pattern of GmKTI1-like in different soybean tissues.Trangenic plants were created using the Agrobacterium-mediated.Following extensive molecular biology research and phenotypic characterization over serveral years,a new transgenic soybean germplasm was identified.This germplasm,which carries the target genes and exhibits genetic stability,was evaluated for its significant application potential.The evaluation included assessments of its insecticial proper ties and measurements of trypsin inhibitor enzyme activity.[Result]GmKTI1-like was located on chromosome 1,and its encoded GmKTI1-like contained one KTI structural domain and one transmembrane structural domain,and the results of subcellular localization showed that GmKTI1-like was a membrane protein.Fluorescence quantitative PCR showed that GmKTI1-like had the highest expression in soybean leaves.Compared with the wild type(WT),the transgenic soybean plants showed significantly enhanced resistance to insect and high levels of trypsin inhibitor under Lamprosema indicata borer stress.[Conclusion]The overexpression of GmKTI1-like transgenic soybean lines significantly increase theresistance to insect,indicating its pivotal role in the soybean defense mechaism against the L.indicata borer.关键词
大豆/豆卷叶螟/胰蛋白酶抑制剂/GmKTI1-likeKey words
soybean/Lamprosema indicata/trypsin protease inhibitor/GmKTI1-like引用本文复制引用
谭玉荣,陈东亮,杨守臻,赖振光,唐向民,孙祖东,曾维英..大豆抗豆卷叶螟GmKTI1-like的功能研究[J].生物技术通报,2025,41(6):99-108,10.基金项目
广西自然科学基金‒青年科学基金项目(2022GXNSFBA035459),广西科技重大专项(桂科AA23062017),广西农科院稳定资助科研团队项目(桂农科2021YT054) （2022GXNSFBA035459）
