生物技术通报2025，Vol.41Issue(6)：130-143,14.DOI:10.13560/j.cnki.biotech.bull.1985.2024-1285
125份马铃薯种质资源遗传多样性及主成分分析
Genetic Diversity and Principal Component Analysis of 125 Potato Germplasm Resources
摘要
Abstract
[Objective]To clarify the genetic relationship between potato germplasm resources,and to provide a research basis for the creation of new germplasm.[Method]In this paper,125 potato varieties(lines)were used as research materials.Morphological markers and SSR molecular markers were used to analyze the differences among germplasm resources,and principal component analysis was used to evaluate the genetic diversity.[Result]The coefficient of variation of the eight phenotypic traits ranged from 19.40%to 63.90%,and the diversity index ranged from 0.990 7 to 1.001 0.According to phenotypic traits,the tested materials could be divided into four categories,most of which were clustered according to phenotypic index characteristics.Twenty-seven pairs of polymorphic SSR primers were screened,and 910 alleles and 832 polymorphic sites were amplified,with a polymorphism site ratio of 90.93%.The average Nei's genetic diversity(H)and Shannon's index(I)were 0.228 6 and 0.362 1,respectively.According to SSR test results,the test materials were clustered into five categories.Most of the varieties were clustered according to their geographical origin,and the new strains were clustered according to their parents.PCA analysis of SSR marker clusters showed that the coordinated distribution results of each group were basically consistent with the clustering results,and the two analysis results supported each other.[Conclusion]There are differences in the classification of groups between phenotypic traits and SSR marker clustering results,but some potato materials show consistent in the two clustering methods,and the materials from the same geographical origin are clustered into one group.Phenotypic traits are affected by environmental conditions and human factors,and cannot reflect all genomic information,but molecular markers directly detect molecular differences in the genome.The combination of the two methods should be used in the analysis and evaluation of genetic diversity of potato germplasm resources,which may provide reference for potato germplasm innovation and genetic improvement.关键词
马铃薯/种质资源/表型性状/SSR标记/遗传多样性Key words
potato/germplasm resources/phenotypic traits/SSR marker/genetic diversity引用本文复制引用
段永红,杨欣,于冠群,夏俊俊,宋陆帅,白小东,彭锁堂..125份马铃薯种质资源遗传多样性及主成分分析[J].生物技术通报,2025,41(6):130-143,14.基金项目
山西省基础研究计划项目(202203021211272),山西省重点研发计划项目(202102140601004),山西农业大学农学院育种工程专项(YZ2021-04),马铃薯遗传改良与种质创新山西省重点实验室项目(202304010921003),山西省现代农业产业技术体系建设专项资金(2025CYJSTX06-11) （202203021211272）
