生物技术通报2025，Vol.41Issue(6)：155-166,12.DOI:10.13560/j.cnki.biotech.bull.1985.2024-1144
CsGR-RBP3克隆及其在采后黄瓜耐冷性中的功能
Cloning of CsGR-RBP3 and Its Functional Roles in Cold Tolerance of Harvested Cucumber
摘要
Abstract
[Objective]Cucumber(Cucumis sativus L.)is a cold-sensitive vegetable prone to chilling injury during postharvest storage,which limits the application of cold storage technology in postharvest cucumbers.Cold treatments induce the expression of CsGR-RBP3(glycine-rich RNA-binding protein 3)in harvested cucumber fruit.Cloning CsGR-RBP3 and studying its functional roles in chilling tolerance of postharvest cucumber can provide candidate gene for the cultivation of new cold-resistant cucumber varieties.[Method]The coding sequence of CsGR-RBP3 was cloned using cDNA from cucumber peels as templates.Virus-induced gene silencing(VIGS)technology was used to knock-down CsGR-RBP3 expression in postharvest cucumbers to study the effects of the reduced expression on chilling injury in cold-stored cucumbers.RNA-Seq was used to identify potential pathways modulated by CsGR-RBP3 regulating chilling tolerance of cucumber fruit.[Result]Cucumber CsGR-RBP3 encoded a protein spanning 168 amino acid residues with a conserved RRM(RNA-recognition motif)domain,which might be a mitochondria-related protein and had low sequence similarity with Arabidopsis AtGR-RBPs.Reducing cold-inducible CsGR-RBP3 expression aggravated chilling injury of postharvest cucumbers during cold storage,downregulated the expression of cold-inducible genes,and reduced mitochondrial antioxidant enzyme activity and gene expression.Furthermore,differentially expressed genes(DEGs)potentially regulated by CsGR-RBP3 were significantly enriched in the phenylalanine metabolism,phenylpropanoid biosynthesis and plant-pathogen interaction pathways.Moreover,the expressions of genes in these three pathways were overall downregulated when cold-inducible expression of CsGR-RBP3 was restricted.These results indicated that the CsGR-RBP3 expression was positively correlated with cold resistance in cold-stored cucumbers,and it might maintain mitochondrial redox balance by regulating activities of mitochondrial-related antioxidant enzymes and integrating multiple defense pathways in cucumber fruit.[Conclusion]CsGR-RBP3 gene had important roles in cold tolerance of harvested cucumber fruit,which may might regulate cold tolerance through multiple defense pathways.关键词
黄瓜/冷害/耐冷性/富含甘氨酸RNA结合蛋白/防御途径Key words
cucumber/chilling injury/chilling tolerance/glycine-rich RNA-binding protein/defense pathway引用本文复制引用
王斌,李健荣,占朝霞,袁晓..CsGR-RBP3克隆及其在采后黄瓜耐冷性中的功能[J].生物技术通报,2025,41(6):155-166,12.基金项目
广东省教育厅重点科研平台和项目(2024GCZX006),广东省基础与应用基础研究基金(2020A1515010068) （2024GCZX006）
