生物技术通报2025，Vol.41Issue(6)：191-207,17.DOI:10.13560/j.cnki.biotech.bull.1985.2024-1146
油茶HD-Zip基因家族鉴定及其在非生物胁迫下的表达分析
Identification of HD-Zip Gene Family in Camellia oleifera and Analysis of Its Expression under Abiotic Stress
摘要
Abstract
[Objective]To identify the HD-Zip gene family members in Camellia oleifera,analyze their structure,characteristics and functions,as well as examine their expression patterns under abiotic stress,including salt,drought,low temperature and high temperature.[Method]Based on the whole genome data of hexaploid common C.oleifera,members of CoHDZs gene family were screened and identified by bioinformatics method.The analysis included an examination of gene structure,conserved motifs,cis-acting elements,chromosomal locations,and gene colinearity.Additionally,expression patterns of CoHDZ genes were analyzed during various developmental stages of C.oleifera,including leaves,buds,petals,pistils,seeds,calyxes,stamens,and fruits.Furthermore,real-time fluorescence quantitative PCR was utilized to investigate the transient expression characteristics of CoHDZs under different stress conditions.[Result]The CoHDZ gene family comprises 46 members(CoHDZ1-CoHDZ46)that are categorized into four distinct subfamilies:HD-Zip Ⅰ,HD-Zip Ⅱ,HD-Zip Ⅲ,and HD-Zip Ⅳ.Members of the CoHDZ genes within the same subfamily have similar structural characteristics.Analysis of cis-acting elements indicated that members of the C.oleifera HD-Zip gene family are significantly involved in light response,hormone response,and abiotic stress response.Chromosomal localization and collinearity analysis revealed that the 46 CoHDZ genes are unevenly distributed across 26 chromosomes and 2 chromosome scaffolds.Whole genome duplication(WGD)events or segmental duplications are likely the primary drivers of the evolution of CoHDZ genes.Transcriptome analysis demonstrated that CoHDZ genes have pronounced tissue-specific expression.Real-time fluorescence quantitative PCR experiments confirmed that members of the C.oleifera HD-Zip gene family are highly expressed under conditions of salt,drought,low temperature,and high temperature stress.Furthermore,the expressions of different CoHDZ genes vary under the same stress conditions,and the expression of the same gene also differs across various stressors.Notably,CoHDZ1,CoHDZ3,CoHDZ14,CoHDZ18,CoHDZ20,CoHDZ22,CoHDZ42,CoHDZ44,and CoHDZ45 are identified as key genes in the C.oleifera HD-Zip family responding to abiotic stress.[Conclusion]CoHDZs genes are widely involved in the growth and development,hormone signaling and abiotic stress response of C.oleifera,and play an important regulatory role.关键词
油茶/HD-Zip基因家族/组织特异性/非生物胁迫Key words
Camellia oleifera/HD-Zip gene family/tissue specificity/abiotic stress引用本文复制引用
黄丹,彭兵阳,张盼盼,焦悦,吕佳斌..油茶HD-Zip基因家族鉴定及其在非生物胁迫下的表达分析[J].生物技术通报,2025,41(6):191-207,17.基金项目
安徽省自然科学基金青年项目(2208085QC92),安徽省高等学校科学研究项目(2024AH050484),省级大学生创新创业训练计划项目(S202310364160),校级大学生创新创业训练计划项目(X202410364499) （2208085QC92）
