时珍国医国药2025，Vol.36Issue(10)：1801-1806,6.DOI:10.70976/j.1008-0805.SZGYGY-2025-1001
祛脂愈肝汤对非酒精性脂肪性肝炎细胞脂质代谢及IL-17表达的影响
Effect of Quzhi Yugan Decoction(祛脂愈肝汤)on lipid metabolism and IL-17 expression in non-alcoholic steatohepatitis cells
摘要
Abstract
Objective To observe the effect of QuzhiYugan Decoction(祛脂愈肝汤,QZYGD)on lipid metabolism and IL-17 expres-sion in a non-alcoholic steatohepatitis(NASH)cell model.Methods The NASH model was established by 1mmol/L free fatty acid(FFA)-induced mixture.Cells were divided into the following groups:the blank group,the model group,the silence group(100 μl blank rabbit serum),low/medium/high-dose QZYGD groups(100 μl of 5％,10％,and 15％medicated serum,respectively),and the polyene phosphatidylcholine group(100 μl of 10％medicated serum).Oil Red O staining was used to observe staining conditions of lipid droplets in NASH cells.RT-qPCR and Western blot(WB)were used to detect the expression of IL-17 mRNA and protein.Re-sults Cells in the blank control group exhibited clear structures,blue nuclei,and no significant orange-red lipid droplets.The model group showed numerous orange-red lipid droplets of varying sizes in the cells.Lipid droplet content in the silence and drug intervention groups was significantly reduced compared to the model group.Compared with the model group,the expression of IL-17 protein and mRNA were significantly decreased in the silence group,the low/medium/high-dose QZYGD groups and the polyene phosphatidylcho-line group(P＜0.01),with the high-dose QZYGD group showing a more significant reduction.Conclusion QZYGD may exert protec-tive effects on NASH cells by inhibiting the expression of the inflammatory factor IL-17,alleviating inflammatory responses,and ameli-ating lipid metabolism.关键词
非酒精性脂肪性肝炎/祛脂愈肝汤/白细胞介素17/脂质代谢Key words
Non-alcoholic steatohepatitis(NASH)/QuzhiYugan Decoction(祛脂愈肝汤)/Interleukin-17/Lipid metabolism分类
医药卫生引用本文复制引用
李开楊,郭震凡,田新材,赵琦,黄敬,杨梅..祛脂愈肝汤对非酒精性脂肪性肝炎细胞脂质代谢及IL-17表达的影响[J].时珍国医国药,2025,36(10):1801-1806,6.基金项目
国家自然科学基金(82160866) （82160866）
贵州省科技计划项目(黔科合基础-ZK[2023]一般433) （黔科合基础-ZK[2023]一般433）
贵州省卫生健康委科学技术基金资助项目(gzwkj2023-208) （gzwkj2023-208）
贵州省教育厅自然科学研究项目(黔教技[2023]017号) （黔教技[2023]017号）
贵州省普通高等学校青年科技人才成长项目(黔教合KY字[2022]263号) （黔教合KY字[2022]263号）
