时珍国医国药2025，Vol.36Issue(10)：1816-1822,7.DOI:10.70976/j.1008-0805.SZGYGY-2025-1003
基于IL-6/STAT3通路探讨内异康复片改善子宫腺肌病纤维化作用的研究
Study on the mechanism of Neiyi Kangfu Tablet(内异康复片)in ameliorating fibrosis of ade-nomyosis based on the IL-6/STAT3 pathway
摘要
Abstract
Objective To investigate the effect of Neiyi Kangfu Tablet(内异康复片,NYKFT)in mouse on fibrosis of adenomyosis and explore its regulatory mechanism on the IL-6/STAT3 signaling pathway.Methods A mouse model of adenomyosis fibrosis was estab-lished by drip feeding of tamoxifen citrate.Seventy-day-old mice were treated with low/medium/high-dose of NYKFT or dienogest.Thermal pain thresholds were compared at different time periods during gavage.After 8 weeks of treatment,samples were collected for histopathological analysis using HE and Masson staining.Western blot(WB)was used to detect the protein expression contents of STAT3 and p-STAT3 in the uterine tissues,and ELISA was used to determine the levels of IL-6,TGF-β1,MCP-1 and MMP9 in serum.Results Compared with the blank group,the model group showed significantly decreased thermal pain thresholds(P＜0.05),increased HE staining histopathological scores and collagen fiber deposition area,elevated p-STAT3/STAT3 relative expression levels(P＜0.05)with statistically significance,and higher concentrations of IL-6,TGF-β1,MMP9,and MCP-1.Compared with the model group,all treatment groups exhibited improved thermal pain thresholds,reduced HE staining histopathological scores and collagen fiber deposition area,and decreased p-STAT3/STAT3 relative expression levels and concentrations of IL-6,TGF-β1,MMP9,and MCP-1.Conclusion The intervention of NYKFT significantly alleviates fibrosis of adenomyosis in the model mice,and its mechanism may be associated with the regulation of the IL-6/STAT3 signaling pathway.关键词
子宫腺肌病/纤维化/内异康复片/他莫昔芬/清湿化瘀法Key words
Adenomyosis/Fibrosis/Neiyi Kangfu Tablet(内异康复片)/Tamoxifen/Therapy of clearing dampness and resol-ving stasis分类
医药卫生引用本文复制引用
邵钰,程文秀,魏绍斌,周思韵..基于IL-6/STAT3通路探讨内异康复片改善子宫腺肌病纤维化作用的研究[J].时珍国医国药,2025,36(10):1816-1822,7.基金项目
国家自然科学基金面上项目(81273796) （81273796）
