时珍国医国药2025，Vol.36Issue(10)：1823-1829,7.DOI:10.70976/j.1008-0805.SZGYGY-2025-1004
基于PI3K/AKT/PTEN信号通路探讨补中益气汤调控Th17细胞免疫代谢改善AIT的作用机制
Exploring the mechanism of Buzhong Yiqi Decoction(补中益气汤)in relieving autoimmune thyroiditis by regulating Th17 immunometabolism based on the PI3K/AKT/PTEN signaling pathway
摘要
Abstract
Objective To explore the mechanism of Buzhong Yiqi Decoction(补中益气汤,BZYQD)in regulating abnormal immune me-tabolism of Th17 cells to relieve autoimmune thyroiditis(AIT)based on the PI3K/AKT/PTEN signaling pathway.Methods 40 NOD.H-2h4 mice were randomly divided into the blank group,the model group,the BZYQD group(9.56 g·kg-1)and the Western medicine group(selenium yeast tablet,3.033×10-5 g·kg-1).The blank group was gavaged with distilled water,and the remaining groups were gavaged with 0.05％aqueous sodium iodide.Each drug administration group was gavaged with liquid medication,while the blank group and the model group were gavaged with equal volume distilled water for 8 weeks.Histopathological changes and lymphocyte infiltration in thyroid gland were observed by HE,serum TGAb and TPOAb were detected by ELISA,PI3K,p-PI3K,AKT,p-AKT,PTEN,GLUT1,PKM2,HK2,and LDHA by Western blot(WB),and IL-17 and RORyt and the localized expression levels of IL-17 and RORγt in thyroid tissues was measured by immunohistochemistry(IHC).Results Compared with the blank group,the model group ex-hibited significant lymphocyte infiltration in the thyroid tissues under light microscopy,a marked increase in serum TGAb and TPOAb levels(P＜0.01),a significant elevation in protein expression of p-PI3K/PI3K,p-AKT/AKT,GLUT1,HK2,PKM2,and LDHA in the thyroid tissues(P＜0.01),and a notable decrease in PTEN protein expression(P＜0.01).Additionally,the localization expres-sion of RORγt and IL-17 was significantly increased.Compared with the model group,all treatment groups showed significantly reduced lymphocyte infiltration in the thyroid tissues,a significant decrease in serum TGAb and TPOAb levels(P＜0.01),a significant reduc-tion in protein expression of p-PI3K/PI3K,p-AKT/AKT,GLUT1,HK2,PKM2,and LDHA in the thyroid tissues(P＜0.05,P＜0.01),and a significant increase in PTEN protein expression(P＜0.01).Furthermore,the localization expression of RORyt and IL-17 was significantly decreased.Conclusion BZYQD can ameliorate the imnmunometabolic abnormality of Th17 cells in AIT,possibly through the PI3K/AKT/PTEN-glycolysis axis as its intervention mechanism.关键词
补中益气汤/自身免疫性甲状腺炎/PI3K/AKT/PTEN信号通路/免疫代谢/Th17细胞Key words
Buzhong Yiqi Decoction(补中益气汤)/Autoimmune thyroiditis/PI3K/AKT/PTEN/Immunometabolism/Th17 cells分类
医药卫生引用本文复制引用
李佳韵,刘子玉,赵卓,金哲,陈怡然,王智民,陈丝,曹慧敏,杨潇..基于PI3K/AKT/PTEN信号通路探讨补中益气汤调控Th17细胞免疫代谢改善AIT的作用机制[J].时珍国医国药,2025,36(10):1823-1829,7.基金项目
国家自然科学基金面上项目(82274455) （82274455）
国家自然科学基金青年基金项目(82104805) （82104805）
辽宁省教育厅重点攻关项目(JYTZD2023196) （JYTZD2023196）
辽宁省科技厅联合基金项目(2023-MSLH-154) （2023-MSLH-154）
尹远平全国名老中医专家传承工作室(202205) （202205）
辽宁省高校基本科研项目储备项目(2024-YJTCB-046) （2024-YJTCB-046）
辽宁省科技计划联合计划(技术攻关计划项目)(2024JH2/102600171) （技术攻关计划项目）
