生物技术通报2025，Vol.41Issue(6)：256-268,13.DOI:10.13560/j.cnki.biotech.bull.1985.2024-1105
灰毡毛忍冬bHLH转录基因家族的鉴定与表达分析
Identification and Expression Analysis of bHLH Transcription Gene Family in Lonicera macranthoides
摘要
Abstract
[Objective]To study the structure and function of bHLH transcription factor family members in Lonicera macranthoides,and to analyze their expression characteristics,so as to lay a foundation for further study on the function of bHLH gene family in flower development of L.macranthoides.[Method]Based on the flower transcriptome data of two varieties of L.macranthoides,the members of bHLH gene family were screened,and bioinformatics analysis was carried out by online tools to obtain the genes with significant expression differences between the two varieties of L.macranthoides.The expression of bHLH was verified by RT-qPCR.[Result]A total of 55 bHLH transcription factor family members were identified.Phylogenetic tree analysis showed that the bHLH protein of L.macranthoides was highly conserved with Arabidopsis thaliana,and the functions of some bHLH proteins were predicted based on the phylogenetic tree.Expression analysis showed that there were significant differences in bHLH genes between the two varieties of L.macranthoides.LmbHLH4/21/35 was highly expressed in the'Changgui',and LmbHLH25 was highly expressed in the flowering stage 5-7 of'Xianglei'.The results of RT-qPCR showed that the relative expression was basically consistent with the transcriptome sequencing data,which confirmed the reliability of the transcriptome sequencing results.[Conclusion]LmbHLH4/21/15/25/35 may be a key transcription factor involved in the regulation of flower growth and development of L.macranthoides,which provides a reference for analyzing the molecular mechanism of flower difference phenotype of two varieties of Lonicera macranthoides.关键词
灰毡毛忍冬/bHLH基因家族/生物信息学/表达分析Key words
Lonicera macranthoides/bHLH gene family/bioinformatics/expression analysis引用本文复制引用
瞿美玲,周思敏,张惊宇,何佳蔚,朱佳源,刘笑蓉,童巧珍,周日宝,刘湘丹..灰毡毛忍冬bHLH转录基因家族的鉴定与表达分析[J].生物技术通报,2025,41(6):256-268,13.基金项目
国家自然科学基金项目(82373992),国家现代农业产业技术体系(CARS-21),湖南省中药材产业技术体系专项(HARS-11),湖南省自然科学基金(2023JJ60477),2020年湖南省一流专业建设点:中药资源与开发,湖南中医药大学重点学科中药学科(校行发规字[2023]2号),2024年湖南省研究生科研创新项目(CX20240739),湖南中医药大学校级科研课题(2022YYZK003) （82373992）
