生物技术通报2025，Vol.41Issue(6)：269-283,15.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0074
灰杨PcAHL17负调控拟南芥的镉耐受性
Populus×canescens AHL17 Negatively Regulates Tolerance to Cadmium in Arabidopsis thaliana
摘要
Abstract
[Objective]We delved into the molecular mechanisms by which AHL17(AT-hook motif nuclear-localized proteins,AHLs)in Populus×canescens regulates the plant's response to cadmium stress.The findings of this research offered a reference for the breeding of stress-resistant varieties and the application of ecological restoration.[Method]Wild-type(WT),vector control(VC),and Arabidopsis thaliana overexpressing PcAHL17(PcAHL17-OE1,PcAHL17-OE,and PcAHL17-OE3)were used as experimental materials,the mechanism of PcAHL17 regulating plant response to cadmium stress was studied.[Result]After cadmium treatment,the expressions of PcAHL17 in the roots,stems,and leaves of Populus×canescens changed.The germination rate and root length of the overexpressed lines showed a significant decrease compared to those of WT and VC.The activity and transcription level of antioxidant enzymes in the overexpressing strains both increased,but still were lower than those in WT and VC.The degree of membrane damage in the overexpressing strains was significantly higher than that in WT and VC,and the accumulation of Cd2+and H2O2 in roots,as well as the influx of Cd2+,were significantly higher than those in WT and VC.Overexpressed strains had lower chlorophyll content,chlorophyll fluorescence parameters,and photosynthetic rate than WT and VC.[Conclusion]The overexpression of PcAHL17 gene may negatively regulate tolerance of transgenic Arabidopsis thaliana to cadmium.关键词
灰杨/镉胁迫/AT-hook核定位蛋白/PcAHL17/离子平衡/活性氧Key words
Populus×canescens/cadmium stress/AT-hook motif nuclear localized proteins/PcAHL17/ion homeostasis/reactive oxygen species引用本文复制引用
冯冰,闫彩霞,刘艺,董凯悦,赵楠,赵瑞,陈少良..灰杨PcAHL17负调控拟南芥的镉耐受性[J].生物技术通报,2025,41(6):269-283,15.基金项目
国家自然科学基金项目(32371828,32071730) （32371828,32071730）
