生物技术通报2025，Vol.41Issue(6)：335-343,9.DOI:10.13560/j.cnki.biotech.bull.1985.2024-1126
糙皮侧耳覆土栽培对土壤中抗生素抗性基因的影响
Effect of Soil-casing Cultivation of Pleurotus ostreatus on Antibiotic Resistance Genes in Soil
摘要
Abstract
[Objective]To investigate the feasibility of reducing the abundance of antibiotic resistance genes(ARGs)in soil using edible fungi soil-casing cultivation mode.[Method]The abundance of ARGs and mobile genetic elements(MGEs)in soil under soil-casing cultivation mode of Pleurotus ostreatus was investigated by high-throughput fluorescence quantitative PCR.The differences in soil bacterial communities before and after the formation of fruiting bodies were analyzed by using 16S rRNA sequencing.Co-occurrence patterns among ARGs,MGEs and bacterial communities were analyzed by using network analysis.[Result]After P.ostreatus cultivation,the total relative abundance of ARGs decreased by 34.62%(P<0.01),and the total absolute abundance decreased by 48.56%(P<0.01),with significant reductions observed in aminoglycoside,sulfonamide,β-lactamase,macrolide-lincosamide-streptogramin B,and tetracycline ARGs.The total relative abundance of MGEs decreased by 20.63%,and the total absolute abundance decreased by 32.99%(P<0.01).Bacterial community structure changed significantly(P<0.01),explaining 31.50%of the variation in ARGs,while the combined effect of bacterial community structure and MGEs explained 8.01%of the variation.[Conclusion]The proliferation and development of P.ostreatus mycelium in the soil changed the structure of microbial community and achieved the effect of reducing the abundance of ARGs in the soil.The method of using edible fungi soil-casing cultivation is easy to operate and requires no high temperature or fermentation treatment,which not only reduces the abundance of ARGs in the soil,but also enables the harvesting of edible mushroom,providing a new perspective for bioremediation of agricultural soils contaminated with ARGs.关键词
抗性污染/农业土壤/食用真菌/微生物群落结构/生物修复Key words
resistance contamination/agricultural soil/edible fungi/microbial community structure/bioremediation引用本文复制引用
弥春霞,许澍,王守现,刘宇,宋庆港,宋爽..糙皮侧耳覆土栽培对土壤中抗生素抗性基因的影响[J].生物技术通报,2025,41(6):335-343,9.基金项目
国家重点研发计划课题(2023YFD2201803),北京市农林科学院创新能力建设项目(KJCX20230102) （2023YFD2201803）
