生物技术通报2025，Vol.41Issue(6)：355-366,12.DOI:10.13560/j.cnki.biotech.bull.1985.2024-1180
碳源对产甲烷菌群调控及产甲烷能力的影响
Effect of Carbon Source in Methanogenic Communities Regulation and Methane Production Capacity
摘要
Abstract
[Objective]Methanogens play a pivotal role in methanogenesis during anaerobic digestion.To obtain an efficient methanogenic microbial community,the regulatory effects of different carbon sources on the methane production potential,methanogen abundance,and microbial community structure were investigated.[Method]Three distinct carbon source culture media(Group A:CH3COONa and CH3OH;Group B:CH3COONa;Group C:CH3COONa and HCOONa)were designed to enrich methanogenic communities by inoculating with anaerobic digestion liquid from kitchen waste(FW)and activated sludge suspension(SS).The performance and diversity composition of methanogenic microbial community were analyzed using gas chromatography,real-time quantitative PCR(qPCR),fluorescence microscopy,and amplicon sequencing.[Result]The microbial communities enriched by carbon sources A,B,and C demonstrated a descending order of methane yield when inoculated with the same inoculum.Group 1A-2 achieved the highest cumulative methane yield over 49 d,with a methane yield of 35.9 mL per gram of available carbon(C)in a unit volume(L)of culture medium.The copy number of mcrA gene and fluorescence microscopy observations indicated a successive decrease in methanogen abundance in communities enriched by carbon sources A,B,and C.Specifically,carbon source A enriched methylotrophic methanogens,while carbon source B significantly boosted the relative abundance of aceticlastic Methanothrix compared to the other groups.Carbon source C notably enhanced the relative abundance of hydrogenotrophic methanogens.[Conclusion]The type of carbon source significantly influence the diversity,richness,and community structure of methanogens and bacteria in the enriched microbial communities.The microbial communities enriched with CH3COONa and CH3OH(Group A)showed the highest methanogen abundance,mainly including Methanothrix,Methanoculleus,Methanomethylovorans,Candidatus Methanoplasma and Methanosarcina,and demonstrated the highest methanogenic capacity.Carbon source A promotes methane(CH₄)production by enriching syntrophic bacteria,balancing the metabolic pathway distribution of methanogens,and facilitating direct interspecies electron transfer(DIET).关键词
厌氧培养/产甲烷菌/实时荧光定量PCR/荧光观察/扩增子测序Key words
anaerobic culture/methanogens/real-time quantitative PCR/fluorescence observation/amplicon sequencing引用本文复制引用
安苗苗,蔺祥淏,梁瑞娜,赵国柱..碳源对产甲烷菌群调控及产甲烷能力的影响[J].生物技术通报,2025,41(6):355-366,12.基金项目
教育部产学合作协同育人项目(2021HXFWSWXY006) （2021HXFWSWXY006）
