土壤学报2025，Vol.62Issue(3)：857-869,13.DOI:10.11766/trxb202401110021
紫云英还田对红壤水稻土氨氧化微生物功能基因丰度的影响
Effects of Chinese Milk Vetch Incorporation on the Gene Abundance of Ammonia-oxidizing Microorganisms in Red Paddy Soil
摘要
Abstract
[Objective]Nitrification,an important component of nitrogen cycling,has the potential to influence soil nitrogen availability.As a result,it will lead to ecological and environmental issues such as eutrophication,and greenhouse gas(nitrous oxide)emissions.The objective of this study was to explore the effect of Chinese milk vetch incorporation on the gene abundance of ammonia-oxidizing microorganisms in red paddy soil under a rice-Chinese milk vetch planting system.[Method]In a field experiment,five fertilization treatments were applied,including Chinese milk vetch incorporation(G),100%chemical fertilizer(C),Chinese milk vetch incorporation+100%chemical fertilizer(GC),and Chinese milk vetch incorporation+20%chemical fertilizer reduction(GCT20),with a no fertilization treatment serving as the control(CK).Real-time quantitative PCR was used to determine the abundance of ammonia-oxidizing archaea(AOA),ammonia-oxidizing bacteria(AOB),and comammox Nitrospira Clade A and Clade B in each treatment.[Result]The results showed that Chinese milk vetch incorporation increased soil organic carbon and total nitrogen content compared with CK,while the 100%chemical fertilizer treatment showed no significant effect.There was no significant difference in AOA and Clade B abundance among all the treatments,indicating a weak response to nitrogen inputs from both chemical fertilizers and green manure.However,both the sole application of chemical fertilizer and its combination with Chinese milk vetch incorporation significantly increased the abundance of AOB and Clade A.Additionally,Chinese milk vetch incorporation alone had no significant effect on AOB and Clade A,suggesting their sensitivity to chemical fertilizer,but resistance to green manure nitrogen input.Furthermore,gene abundance of all ammonia-oxidizing microorganisms showed a similar trend across growth stages,with higher abundance observed during the mature stage followed by the booting stage and then the tillering stage.This suggests that growth stage and oxygen concentration are key factors influencing the growth of these microorganisms in paddy soils.[Conclusion]Growth stage is a key factor affecting the abundance of ammonia-oxidizing microorganisms in paddy soils.Additionally,Chinese milk vetch incorporation had a weaker promotion effect on AOB and Clade A abundance compared to chemical fertilizer,potentially contributing to nitrogen retention and stability in paddy soils.关键词
红壤水稻土/绿肥/氨氧化古菌/氨氧化细菌/完全氨氧化菌Key words
Red paddy soil/Green manure/Ammonia-oxidizing archaea/Ammonia-oxidizing bacteria/Comammox Nitrospira分类
农业科技引用本文复制引用
孙鲁沅,刘佳,冯蒙蒙,刘晗,马停停,陈晓芬,林永新..紫云英还田对红壤水稻土氨氧化微生物功能基因丰度的影响[J].土壤学报,2025,62(3):857-869,13.基金项目
国家重点研发计划项目(2021YFD1700203)、国家自然科学基金项目(42267046,32160766)和国家现代农业产业技术体系项目(CARS-22)资助Supported by the National Key Research and Development Program of China(No.2021YFD1700203),the National Natural Science Foundation of China(Nos.42267046 and 32160766),the Agriculture Research System of MOF and MARA,China(No.CARS-22) （2021YFD1700203）
