天津科技大学学报2025，Vol.40Issue(3)：10-19,10.DOI:10.13364/j.issn.1672-6510.20240088
阪崎克罗诺杆菌基因ESA_01668的功能及机制研究
Function and Mechanism Study of Gene ESA_01668 in Cronobacter sakazakii
摘要
Abstract
Cronobacter sakazakii has strong desiccation tolerance,but the specific molecular mechanism of its desiccation resistance is still unclear.Previous studies in our laboratory found that the disruption of the gene ESA_01668,whose encoded product is a hypothetical protein,resulted in a significant decrease in the drying resistance of the strain.In order to further explore the function of the gene ESA_01668,the mutant strain ΔESA_01668 was constructed by homologous recombination,and the complement strain cpESA_01668 was constructed by vector pACYC184.By comparing the physiological character-istics of wild-type WT,ΔESA_01668 and cpESA_01668,it was found that the gene ESA_01668 had no significant effect on the growth of the strain under normal condition,but promoted the strain growth under high salt condition.The deletion of the gene ESA_01668 resulted in a decrease in the desiccation resistance,motility,biofilm formation and membrane hydrophobic-ity,an increase in the membrane permeability,and an inhibition on the transport of proline and transport/synthesis of betaine.This study has provided a reference for further elucidating the desiccation resistance related function of the gene ESA_01668 in C.sakazakii.关键词
阪崎克罗诺杆菌/耐干燥/假设蛋白/ESA_01668基因Key words
C.sakazakii/desiccation resistance/hypothetical protein/gene ESA_01668分类
轻工业引用本文复制引用
孙启秀,司默涵,李萍,杜欣军..阪崎克罗诺杆菌基因ESA_01668的功能及机制研究[J].天津科技大学学报,2025,40(3):10-19,10.基金项目
国家重点研发计划项目(2023YFF1103900) （2023YFF1103900）
