天津科技大学学报2025，Vol.40Issue(3)：20-26,36,8.DOI:10.13364/j.issn.1672-6510.20230223
枯草芽孢杆菌碱性蛋白酶的系统生物信息分析
Systematic Bioinformatics Analysis of Alkaline Proteases from Bacillus subtilis
摘要
Abstract
In this present study,the bioinformatic characteristics of Bacillus subtilis were analyzed with the use of such databases as NCBI.The results indicated that G,V,and A amino acids often appeared as conserved sites in alkaline prote-ases.Most alkaline proteases belonged to the peptidase S8 family,including two common types:one is the subtilisin subfam-ily with a length of 379-382 amino acids,an isoelectric point of around 9,an instability index of 11-25,and a fat index of 80-83;the other is S8-1 subfamily with a length of 400-442 amino acids,an isoelectric point of around 5,an instability index between 30-36,and a fat index between 78-80.Both of them are hydrophilic proteins.Moreover,some alkaline proteases contained inhibitory factor i9 superfamily domains.Further analysis of the domain and function of alkaline protease in this study is of great significance for enhancing its industrial application.关键词
碱性蛋白酶/枯草芽孢杆菌/序列比对/生物信息学Key words
alkaline protease/Bacillus subtilis/sequence alignment/bioinformatics分类
生物工程引用本文复制引用
袁宇峰,白琳,毛淑红..枯草芽孢杆菌碱性蛋白酶的系统生物信息分析[J].天津科技大学学报,2025,40(3):20-26,36,8.基金项目
国家自然科学基金项目(32001657),国家重点研发计划项目(2021YFC2100400) （32001657）
