天津科技大学学报2025，Vol.40Issue(3)：37-43,7.DOI:10.13364/j.issn.1672-6510.20240054
鸟苷核苷酸在不同品系卤虫卵中的含量及其对卤虫发育的影响
Content of Guanosine Nucleotide in Different Populations of Artemia Cysts and Its Effect on the Development of Artemia
摘要
Abstract
In order to investigate the content of guanosine nucleotides in different populations of Artemia cysts and its effect on their development,in the present study we analyzed the content of guanosine nucleotides in the cysts of thirteen popula-tions of Artemia in China and Artemia at different development stages by high performance liquid chromatography,especially the content of diguanosine tetraphosphate(Gp4G).The results showed that the contents of guanosine nucleotides had significant differences in cysts of different Artemia populations.The Gp4G content of ten populations was higher than other detected guanosine nucleotides,accounting for 39.49％-56.88％of total guanosine nucleotides.Among them,the Gp4G content of cysts from Aibi Lake,Xinjiang(CHN-SL-71)was highest.Moreover,it was found that the Gp4G content of Ar-temia cysts was related to storage time and hatchability,and the longer storage time of Artemia cysts would lead to the reduc-tion of Gp4G,which would further affect hatchability.Further analysis was also conducted on the changes of the contents of guanosine nucleotides,especially Gp4G and guanosine triphosphate(GTP),in Artemia at different developmental stages.It was found that Gp4G was synthesized during oogenesis,and the content was the highest in Artemia cysts.During the devel-opment process,the content of Gp4G experienced two sharp declines,that is,from cysts to instar Ⅰ nauplii and from instarⅡ nauplii to adults,finally dropped to zero.During this period,GTP content gradually increased.It is speculated that Gp4G is converted into GTP to provide energy for cells during the development of Artemia.The research results provide a new way to evaluate the vitality of Artemia cysts,and lay a foundation for the development of guanosine nucleic acids active prod-ucts(e.g.Gp4G)in Artemia cysts.关键词
卤虫卵/发育/鸟苷核苷酸/四磷酸二鸟苷Key words
Artemia cysts/development/guanosine nucleotide/Gp4G分类
生物学引用本文复制引用
于梦雨,徐思雨,马颖超,高美荣,隋丽英..鸟苷核苷酸在不同品系卤虫卵中的含量及其对卤虫发育的影响[J].天津科技大学学报,2025,40(3):37-43,7.基金项目
山西省运城盐湖保护利用研究院2023年度"揭榜挂帅"研究项目(YHYJ-2023005) （YHYJ-2023005）
