天津师范大学学报（自然科学版）2025，Vol.45Issue(3)：28-34,7.DOI:10.19638/j.issn1671-1114.20250305
绞股蓝总皂苷调控HIF-1α/BNIP3通路改善力竭训练大鼠运动性疲劳的作用机制
Mechanism of gynostemma pentaphyllumsaponins improving exercise-induced fatigue in exhaustive training rats by adjusting HIF-1α/BNIP3 pathway
摘要
Abstract
To explore the mechanism of gynostemma pentaphyllumsaponins(GPs)in ameliorating exercise-induced fatigue,SD rats were used as experimental subjects,and the groups including control group,exhaustive training group(ET),HOL group,L-GPs group,H-GPs group and GPs+2-ME2 group were set up.The rats in control group was fed normally,while the rats in other groups began swimming training on the day of gavage of GPs.Within 24 hours after the last training,the exhausted weight-bearing swimming time of rats was recorded.Serum muscle injury indicators including lactate dehydrogenase(LDH)and creatine kinase(CK)content were detected,and metabolism indicators including glucose(Glu),lactic acid(LA)and urea nitrogen(BUN)content were detected.HE staining was used to detect the pathological morphology of skeletal muscle tissue.The ultrastructure of skeletal muscle was observed under transmission electron microscopy.Oxidative stress indicators in skeletal muscle including superoxide dismutase(SOD),malondialdehyde(MDA)and catalase(CAT)content were detected,and HIF-1 α,BNIP3,LC3-Ⅱ/Ⅰ,and P62 proteins were detected by Western blot.The results illustrated that:① The skeletal muscle membrane structure of control group was clear and the mitochondrial structure was intact.② The arrangement of skeletal muscle tissue in the ET group was irregular,with visible swelling of the muscle interstitium,deformation and rupture of muscle fibers,accompanied by a large amount of inflammatory cell infiltration,a decrease in the number of mitochondria,and great dissolution and loss of mitochondrial cristae.③ The skeletal muscle tissue of the HOL,L-GPs,and H-GPs groups was arranged more neatly,with reduced muscle fiber breakage and a small amount of inflammatory cell infiltration,the num-ber of mitochondria increased,and the mitochondrial cristae were denser and more complete in structure;the arrangement of skeletal muscle tissue in the ET group was irregular,with visible swelling of the muscle interstitium,deformation and rupture of muscle fibers,accompanied by a large amount of inflammatory cell infiltration,a decrease in the number of mitochondria,and great dissolution and loss of mitochondrial cristae.The skeletal muscle tissue of the HOL,L-GPs,and H-GPs groups was arranged more neatly,with reduced muscle fiber breakage and a small amount of inflammatory cell infiltration,the number of mitochondria increased,and the mitochondrial cristae were denser and more complete in structure.④ The muscle fiber rup-ture,inflammatory cell infiltration,and mitochondrial cristae dissolution in the GPs+2-ME2 group further increased,while the number of mitochondria further decreased.⑤ The exhaustion time,LDH,CK,LA,BUN,MDA,and P62 expression levels were higher in ET group than those in the control group,while the expression levels of Glu,SOD,CAT,HIF-1α,BNIP3,and LC3-Ⅱ/Ⅰ were lower than those in the control group(P＜0.05);The exhaustion time,Glu,SOD,CAT,HIF1α,BNIP3,and LC3-Ⅱ/Ⅰ expression levels were higher in the HOL,L-GPs and H-GPs than those in the ET group,while the LDH,CK,LA,BUN,MDA,and P62 expression levels were lower than those in the ET group(P＜0.05).⑥ The expression levels of LDH,CKLA,BUN,MDA,P62 in the GPs+2-ME2 group were higher than those in the H-GPs group,and the exhaustion time,expression levels of Glu,SOD,CAT,HIF-α,BNIP3,LC3-Ⅱ/Ⅰ were lower than those in the H-GPs group(P＜0.05).The above results showed that GPs could improve exercise-induced fatigue in ET rats by activating HIF-1 α/BNIP3 pathway.关键词
绞股蓝总皂苷/低氧诱导因子-1α/HIF-1α/BNIP3通路/运动性疲劳Key words
gynostemma pentaphyllumsaponins(GPs)/hypoxia inducible factor-1α/HIF-1α/BNIP3 pathrway/exercise-induced fatigue分类
中医学引用本文复制引用
高修鹏,张永智,陈宏志..绞股蓝总皂苷调控HIF-1α/BNIP3通路改善力竭训练大鼠运动性疲劳的作用机制[J].天津师范大学学报（自然科学版）,2025,45(3):28-34,7.基金项目
河南省教育科学规划专项课题(2024JZX137) （2024JZX137）
河南省教育厅高等学校重点科研项目资助计划(22A320069). （22A320069）
