生殖医学杂志2025，Vol.34Issue(6)：790-796,7.DOI:10.3969/j.issn.1004-3845.2025.06.010
阴道菌群多样性对宫颈病变的影响
Exploring the effect of vaginal flora diversity in cervical lesions
摘要
Abstract
Objective:To analyze the characteristics of vaginal flora in different cervical lesions by 16S rDNA high-throughput sequencing technology,and to explore the impact of diversity of vaginal microbiota on the progression of cervical lesions. Methods:A total of 168 women admitted to Yinchuan Maternal and Child Health Hospital from December 2022 to March 2024 were selected as the study subjects,and they were divided into cervical high-grade squamous intraepithelial lesions(HSIL,n=46)group,cervical low-grade squamous intraepithelial lesions(LSIL,n=76)group and normal cervical group(CONTROL,n=46).The composition of vaginal flora of three groups was analyzed by 16srDNA high-throughput sequencing technology. Results:The infection rates of HPV16 and HPV18 were highest in the HSIL group,followed by the LSIL group,and lowest in the CONTROL group,with significant differences between groups(P＜0.05).The analysis of vaginal microbiota composition in each group showed that at the phylum level,the abundance of Proteobacteria in the HSIL group was significantly increased compared to the LSIL group and the CONTROL group(P＜0.05).At the class level,the proportion of Proteobacteria in the HSIL group was significantly increased compared to the LSIL group and the CONTROL group(P＜0.05).At the order level,the proportion of Corynebacteriales and Burkholderia in the HSIL group was significantly increased compared to the LSIL group and the CONTROL group(P＜0.05).At the family level,the proportion of Mycobacteriaceae and Burkholderia in the HSIL group was significantly increased compared to the LSIL group and the CONTROL group(P＜0.05).At the genus level,compared with the CONTROL group and LSIL group,the HSIL group showed a significant decrease in Lactobacillus genus(P＜0.05),but a significant increase in Gardnerella genus(P＜0.05).The Shannon,Chao1,and Simpson indices showed that the richness and diversity of vaginal microbiota in the HSIL group were the highest,significantly higher than those in the LSIL group(P＜0.05). Conclusions:The severity of cervical lesions would be related to the disturbance of vaginal microecology.The composition of vaginal microbes in women with cervical HSIL is characterized by a decrease in Lactobacillus and an increase in Gardnerella genus.The improvement of vaginal microecology in patients with HSIL and the increase of Lactobacillus vaginalis may have a regulatory effects on the outcomes of cervical lesions.关键词
阴道菌群多样性/宫颈病变/16Sr DNA高通量测序Key words
Vaginal flora diversity/Cervical lesions/16Sr DNA high-throughput sequencing分类
临床医学引用本文复制引用
张心愿,屈海蓉,许华瑞,陈莉,张洪炜,赵福英..阴道菌群多样性对宫颈病变的影响[J].生殖医学杂志,2025,34(6):790-796,7.基金项目
银川市科技创新专项(2022SF07) （2022SF07）
