生殖医学杂志2025，Vol.34Issue(6)：808-816,9.DOI:10.3969/j.issn.1004-3845.2025.06.012
IL-32通过miR-141-5p/PDGFRA促进宫颈鳞癌的进展
Interleukin-32 in the progress of cervical squamous cell carcinoma through miR-141-5p/PDGFRA
摘要
Abstract
Objective:To explore the role of Interleukin-32(IL-32)in the development of cervical cancer and the related molecular mechanism. Methods:Gene interference and construction of overexpression plasmid were used for knockdown and overexpression of IL-32 level in cells of cervical cancer.Cell proliferation activity was detected by CCK-8 assay,cell migration was detected by scratch healing assay,cell invasion was detected by Transwell assay,gene expression levels of IL-32,miR-141-5p and platelet-derived growth receptor alpha(PDGFRA)in cells were detected by quantitative real time polymerase chain reaction(qRT-PCR)assay,and expression levels of IL-32 protein in tissues were detected by immunohistochemical assay.Bioinformatics methods predicted the target gene relationship between miR-141-5p and PDGFRA. Results:IL-32 was significantly increased in tissues of cervical squamous cell carcinoma and cell lines of cervical cancer(SiHa and HeLa)when compared with those of paracancer tissues and normal cervical epithelial cell line HCerEpiC(all P＜0.05).In cervical cancer cell line SiHa,IL-32 knockdown significantly inhibited cell proliferation,migration and invasion(P＜0.05).In SiHa cell,knockdown or overexpression of IL-32 significantly decreased or increased the expression of miR-141-5p(P＜0.05).Knockdown of miR-141-5p also significantly inhibited the proliferation,migration and invasion of SiHa cell(P＜0.05).Bioinformatics methods predicted that PDGFRA was the target gene of miR-141-5p.Both IL-32 and miR-141-5p could reverse regulate the expression of PDGFRA(P＜0.05). Conclusions:IL-32 is highly expressed in cervical squamous cell carcinoma,which may improve the proliferation,migration and invasion of cancer cells through miR-141-5p/PDGFRA,and promotes the occurrence and development of cervical squamous cell carcinoma.关键词
宫颈鳞癌/白介素-32/miR-141-5p/血小板衍生生长因子受体αKey words
Cervical squamous cell carcinoma/Interleukin-32/miR-141-5p/Platelet-derived growth receptor alpha分类
临床医学引用本文复制引用
刘禹,林晓雨,马海霞,王金娟,李莉,刘建兵..IL-32通过miR-141-5p/PDGFRA促进宫颈鳞癌的进展[J].生殖医学杂志,2025,34(6):808-816,9.基金项目
国家自然科学基金面上项目(82272622) （82272622）
山西省基础研究计划项目(20210302123320,202203021211045) （20210302123320,202203021211045）
