生殖医学杂志2025，Vol.34Issue(6)：826-832,7.DOI:10.3969/j.issn.1004-3845.2025.06.014
基于生物信息学分析子痫前期中miR-943的作用研究
Research on miR-943 in preeclampsia based on bioinformatics analysis
摘要
Abstract
Objective:To conduct bioinformatics analysis of miR-943 target genes related to preeclampsia,so as to provide academic direction for the study of miR-943 in the pathogenesis of preeclampsia. Methods:Based on the dataset GSE 206988 in the public gene chip database GEO,which shows downregulation of miR-943 expression in placental tissue of preeclampsia patients.The corresponding analysis websites miRPathDB and TargetScan were used for target gene prediction.The Venn diagram helped derive intersecting data.Gene ontology(GO)functional annotation analysis was conducted to predict possible target genes with clusterProfiler R package,Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis was carried out to analyze the concentrate signaling pathway,and target gene protein interaction network(PPI)was constructed with the database STRING. Results:TargetScan and miRPathDB predicted 36 crossover target genes.GO functional annotations were enriched to 15 items,with cellular components(CC)mainly enriched in signal recognition granules and endoplasmic reticulum,and molecular functions(MF)mainly enriched in placental growth factor activated receptor activity,protein phosphatase regulatory activity and so on.KEGG pathway analysis showed that the miR-943 target gene was mainly related to signaling pathways such as PI3K Akt,cAMP,MAPK and so on. Conclusions:The target gene function of miR-943 was related to the activation of placental growth factor receptor activity,protein phosphatase regulation activity and so on.The signaling pathways involved were closely related to PE,and miR-943 may be involved in the occurrence and development of PE through the regulation of target genes.关键词
子痫前期/miRNA-943/生物信息学分析Key words
Preeclampsia/miRNA-943/Bioinformatics analysis分类
基础医学引用本文复制引用
沈婕,沈树娜,林元,邓森灵,郑华玲..基于生物信息学分析子痫前期中miR-943的作用研究[J].生殖医学杂志,2025,34(6):826-832,7.基金项目
海南省自然科学基金(822RC853) （822RC853）
