土壤学报2025，Vol.62Issue(3)：740-751,12.DOI:10.11766/trxb202402030060
溶解性有机质介导下Shewanella putrefaciens CN32与Geobacter sulfurreducens PCA共培养对汞形态转化的影响
Effect of Shewanella putrefaciens CN32 and Geobacter sulfurreducens PCA Co-culture on Mercury Transformation Mediated by Dissolved Organic Matter
摘要
Abstract
[Objective]A variety of dissolved organic matter(DOM)and microorganisms affect mercury transformation under anaerobic conditions by mediating biogeochemical cycles.However,it is still unclear how specific organic matter would affect mercury transformation under this condition.[Method]Using batch experiments,the effect of DOM and the co-culture of microorganisms on mercury transformation were investigated.Under simulated the anaerobic environment,three kinds of DOM(cysteine and glutathione containing sulfhydryl and fulvic acid)and two kinds of bacteria(Shewanella putrefaciens CN32 and Geobacter sulfurreducens PCA)were selected to create a reduction environment and methylated mercury,respectively.Batch experiments were carried out to investigate the effect of DOM and the co-culture of microorganisms on mercury transformation.[Result]The results showed that the unit cell adsorption/uptake capacity of G.sulfurreducens PCA for Hg(Ⅱ)was 69.0%of that of S.putrefaciens CN32.Cysteine and glutathione did not alter the proportion of intracellular mercury in G.sulfurreducens PCA,but the proportion of intracellular mercury in S.putrefaciens CN32 was decreased.In the culture of G.sulfurreducens PCA,cysteine promoted mercury reduction and methylation,glutathione promoted mercury reduction,and fulvic acid inhibited mercury reduction and methylation.In the co-culture system of S.putrefaciens CN32 and G.sulfurreducens PCA,the mercury methylation ratio was as high as 18.7%±3.1%after the addition of cysteine complexed mercury,which was mainly attributed to the enhancement of Hg methylation of G.sulfurreducens PCA by microbial co-culture.[Conclusion]The results of this study elucidate the mechanism of DOM and co-culture of microorganisms on mercury species transformation in anaerobic condition,and provide a theoretical basis for the remediation of mercury pollution in flooded paddy field wetland.关键词
汞/溶解性有机质/微生物共培养/甲基化/还原Key words
Mercury/Dissolved organic matter/Microorganisms co-culture/Methylation/Reduction分类
资源环境引用本文复制引用
宋恩,蒋新,王韫橙,江杨昭,赵皓宇,胡大福,胡玉洁,卞永荣,杨兴伦,叶茂..溶解性有机质介导下Shewanella putrefaciens CN32与Geobacter sulfurreducens PCA共培养对汞形态转化的影响[J].土壤学报,2025,62(3):740-751,12.基金项目
国家重点研发计划项目(2022YFC3700805)、国家自然科学基金项目(42077138)和中国科学院战略重点研究项目(XDA28010501)资助Supported by National Key Research and Development Program of China(No.2022YFC3700805),the National Natural Science Foundation of China(No.42077138)and the Strategic Key Research Project of Chinese Academy of Sciences(No.XDA28010501) （2022YFC3700805）
