土壤学报2025，Vol.62Issue(3)：779-790,12.DOI:10.11766/trxb202402170070
一株高效植物促生菌阻控小麦从弱碱性农田土壤中吸收镉的作用
Role of a Highly-Efficient Plant Growth-Promoting Bacterium in Decreasing Cd Uptake of Wheat Plant in Cd-contaminated Weakly Alkaline Arable Soils
摘要
Abstract
[Objective]The safe utilization of farmland with mild to moderate cadmium(Cd)pollution is of great significance for the safety of agricultural products and human health.However,the effects of plant growth-promoting bacteria with the ability to immobilize Cd and regulate Cd-uptake by wheat plant from weakly alkaline farmland soil remains unexploited.[Method]Triticum aestivum L.Yangmai-13 was used as the tested plant,and weakly alkaline heavy metal polluted farmland soil was used as the test soil.Pot experiments were conducted to study the dynamic effects of Pseudomonas taiwanensis WRS8 on wheat growth,Cd content in rhizosphere soil and different parts of wheat,as well as rhizospheric and root endophytic bacterial communities during the jointing,booting,and maturity stages.[Result]The results showed that compared to the control group without inoculation,rhizosphere inoculation with strain WRS8 led to a significant increase in the aboveground biomass,root biomass,and grain biomass of Yangmai 13 by 34%-64%,60%-102%,and 10%-14%,respectively.It decreased the Cd content in shoots,roots,grains,and rhizosphere soil by 55%-60%,5%-8%,78%-82%,and 32%-49%.Moreover,inoculation with strain WRS8 significantly increased the pH value of the rhizosphere soil during the booting and maturity stages.In terms of rhizospheric and root endophytic bacterial communities,inoculation with strain WRS8 only resulted in a significant reduction in the alpha diversity index of endophytic bacterial communities during the booting stage;The principal co-ordinates analysis clustering results showed that both the treatment with live and inactivated bacteria significantly changed the bacterial community structure in the rhizosphere soil and roots,and the bacterial community structure also changed significantly with the extension of the growth period.Inoculating strain WRS8 reduced the relative abundances of Chloroflexi and Proteobacteria in the rhizosphere soil,while increasing the relative abundance of Arthrobacter and Bacillus.Also,strain WRS8 inoculation reduced the relative abundances of Actinobacteria and Chloroflexi phyla of root endophytic bacterial communities,while increasing the relative abundance of Pseudomonas during the jointing and booting stages.[Conclusion]In summary,strain WRS8 not only significantly reduced the absorption of Cd by Yangmai 13 from weakly alkaline farmland soil,but also effectively improves wheat biomass and yield,suggesting its potential to achieve safe utilization of weakly alkaline heavy metal polluted farmland.关键词
镉污染/弱碱性农田土壤/小麦/假单胞菌/生育期Key words
Cadmium contamination/Weakly alkaline arable soil/Wheat plant/Pseudomonas/Growth stage分类
生物科学引用本文复制引用
冯海,张强兵,王琪,吴洪生,程诚..一株高效植物促生菌阻控小麦从弱碱性农田土壤中吸收镉的作用[J].土壤学报,2025,62(3):779-790,12.基金项目
国家自然科学基金项目(42177020)、南京信息工程大学人才启动经费(003342)和国家级大学生创新创业训练计划支持项目(202310300057Z)资助 Supported by the National Natural Science Foundation of China(No.42177020),the Startup Foundation for Introducing Talent of NUIST,China(No.003342),and the National College Student Innovation and Entrepreneurship Training Program Support Project of China(No.202310300057Z) （42177020）
