现代医药卫生2025，Vol.41Issue(6)：1297-1301,5.DOI:10.3969/j.issn.1009-5519.2025.06.001
miR-4435对鼻咽癌细胞生物学行为的影响研究
Effect of miR-4435 on malignant behavior of nasopharyngeal carcinoma cells
摘要
Abstract
Objective To investigate the effect of miR-4435 on the biological behavior of nasopharynge-al carcinoma by regulating the expression of cell cycle associated protein 1(CAPRIN1).Methods The expres-sion levels of miR-4435 in human nasopharyngeal carcinoma cell lines CNE-2Z,C666-1,SUNE-1,HNE-1 and immortalized nasopharyngeal epithelial cell NP69 were detected by fluorescence quantitative polymerase chain reaction.C666-1 cells were transfected with miR-4435 mimics and control mir-nc,and divided into miR-4435 group and miR-NC group.The proliferation and cell cycle changes of c666-1 cells,as well as the expression levels of CAPRIN1 mRNA,CAPRIN1 protein,cyclin dependent kinase 1(CDK1),Cyclin B and Cyclin E were compared between the two groups.The targeted binding of miR-4435 to CAPRIN1 was verified by double lu-ciferase activity report assay.Results Compared with NP69 cells,the relative expression level of miR-4435 in CNE-2Z,c666-1,sune-1 and HNE-1 cells was significantly decreased(F=18.92,P<0.01),and the relative expression level of miR-4435 was most significantly decreased in C666-1 cells(P<0.01).The relative expres-sion levels of miR-4435 in C666-1 cells were(0.19±0.02)and(0.99±0.09)in the miR-NC group and miR-4435 group,respectively,and the difference was statistically significant(t=14.55,P<0.01).Compared with the miR-NC group,the proliferation ability of C666-1 cells in the miR-4435 group was significantly inhibited on day 2,3,4,and 5(P<0.05).Compared with the miR-nc group,the number of C666-1 cells in the G0/G1 phase increased significantly in the miR-4435 group(t=4.69,P<0.01),while the number of cells in the S phase and G2/M phase was significantly decreased(t=4.32,P<0.01;t=4.79,P<0.01).Transfection of miR-4435 significantly reduced the luciferase activity of C666-1 cells containing CAPRIN1 WT(t=13.54,P<0.01).The relative expression levels of CAPRIN1 mRNA in the miR-NC group and miR-4435 group in C666-1 cells were(5.08±0.34)and(1.01±0.09),respectively.The difference between the two groups was statis-tically significant(t=11.54,P<0.01).Compared with the miR-NC group,the protein expression levels of CAPRIN1,CDK1,Cyclin B,and Cyclin E proteins were significantly decreased in the miR-4435 group of C666-1 cells(P<0.01).Conclusion miR-4435 can inhibit the proliferation and cell cycle progression of nasopha-ryngeal carcinoma cells,and act as a tumor suppressor gene by suppressing the expression of CAPRIN1.关键词
鼻咽癌/miR-4435/细胞周期相关蛋白1/细胞生物学Key words
Nasopharyngeal carcinoma/miR-4435/Cell cycle associated protein 1/Cell biology分类
临床医学引用本文复制引用
王挺,张杨,郭洁,范崇盛,蒋伟..miR-4435对鼻咽癌细胞生物学行为的影响研究[J].现代医药卫生,2025,41(6):1297-1301,5.基金项目
国家自然科学基金项目(81760546). （81760546）
