现代医药卫生2025，Vol.41Issue(6)：1302-1306,5.DOI:10.3969/j.issn.1009-5519.2025.06.002
微小病变性肾病与局灶节段性肾小球硬化患者肾组织mRNA表达谱差异研究
Study on the differential expression profile of renal tissue mRNA between patients with minimal change disease and focal segmental glomerulosclerosis
摘要
Abstract
Objective To analyze the differences of renal mRNA expression profiles between patients with minimal change disease(MCD)and focal segmental glomerulosclerosis(FSGS),and screen the differential diagnostic genes between MCD and FSGS.Methods The expression profile data sets of MCD and FSGS from GEO database were collected and divided into test set and validation set.At the same time,they were divided into MCD group(54 cases)and FSGS group(91 cases).The differentially expressed genes were identified by R software,and the enrichment analysis was performed.The diagnostic markers were screened and verified through the test set and validation set.The receiver operating characteristic curve was drawn,and the predic-tive value was evaluated by the area under the curve(AUC).Results Compared with the MCD group,the FSGS group had four different genes,including three up-regulated genes(COLEC12,SFRP2,MOXD1)and one down regulated gene(ENPP6).In the validation set,the relative expression levels of COLEC12,SFRP2 and MOXD1 in the FSGS group were higher than those in the MCD group,and the relative expression level of EN-PP6 in the FSGS group was lower than that in the MCD group,the differences were statistically significant(P<0.05).The AUC values for predicting FSGS in patients of the test set with COLEC12,SFRP2,MOXD1 and ENPP6 expression levels were 0.794,0.825,0.800 and 0.768,respectively.The AUC values for predicting FSGS in patients of the validation set with COLEC12,SFRP2,MOXD1 and ENPP6 expression levels were 0.668,0.635,0.638 and 0.733,respectively.Conclusion COLEC12,SFRP2,MOXD1 and ENPP6 can be used as the key diagnostic markers to distinguish MCD and FSGS.关键词
微小病变性肾病/局灶节段性肾小球硬化/诊断基因Key words
Minimal change nephropathy/Focal segmental glomerulosclerosis/Diagnostic genes分类
药学引用本文复制引用
寇敏,吴芳,王惠,郭琇婷,杨园园,赵丽君..微小病变性肾病与局灶节段性肾小球硬化患者肾组织mRNA表达谱差异研究[J].现代医药卫生,2025,41(6):1302-1306,5.基金项目
山西省基础研究计划(自由探索类)项目(202203021212084). （自由探索类）
