现代医药卫生2025，Vol.41Issue(6)：1307-1310,1314,5.DOI:10.3969/j.issn.1009-5519.2025.06.003
淫羊藿素对非小细胞肺癌的抑制作用研究
Study on the inhibitory effect of icaritins on non-small cell lung cancer
摘要
Abstract
Objective To investigate the inhibitory effect of icaritins on non-small cell lung cancer(NSCLC).Methods Icaritin was mixed into dimethyl sulfoxide and prepared with culture medium at concen-trations of 1.90,3.80,7.60,15.20,and 30.40 μg/mL(respectively for icariin groups A to E).Cisplatin was prepared in the same way at concentrations of 3.38,6.75,12.50,25.00,and 50.00 μg/mL(respectively for groups A to E of cisplatin).Icariin at A concentration of 7.60 μg/mL was added successively to cisplatin at concentrations of 3.38 μg/mL,6.25,12.50,25.00,and 50.00 μg/mL to form combined groups A to E.The effects of icaritins on BEAS-2B cell activity,A549 cell clone formation,and A549 cell apoptosis were analyzed,and the protein expression levels of protein kinase B(AKT),phosphorylated AKT(p-AKT),B-cell lymphoma factor 2(Bcl-2),Bim,and Caspase-9 were compared among icaritins C group,cisplatin B group,and combina-tion B group.Results After treating BEAS-2B cells for 24 and 48 hours,the semi-inhibitory concentrations(IC50)of icaritins group at different levels were(55.25±2.98)and(43.04±2.35)μg/mL,and the IC50 values of cisplatin group at different levels of cisplatin were(10.37±0.90)and(6.70±0.43)μg/mL,The IC50 val-ues of the combination group at different levels were(6.75±0.27)and(5.40±0.58)μg/mL,respectively,and the cell viability rate did not show a significant decrease compared with the cisplatin group at different lev-els(P>0.05).The cell clone formation rate in the combined B group was lower than that in the icaritin C group and cisplatin B group,and the difference was statistically significant(P=0.01).The apoptosis rate of cells in the combined B group was higher than that in the icaritin C group and cisplatin B group,and the differ-ence was statistically significant(P=0.03).The protein expression levels of AKT,p-AKT,and Bcl-2 proteins in the combined B group were lower than those in the icaritin C group and cisplatin B group,and the differ-ences were statistically significant(P=0.03).The protein levels of Bim and Caspase-9 in the combined B group were significantly higher than those in the icaritin C group and cisplatin B group,and the differences were statistically significant(P=0.02).Conclusion Icaritins combined with cisplatin can inhibit the activa-tion of phosphatidylinositol 3-kinase/AKT signaling pathway,regulate the activities of Bcl-2 and Bim protein,and involve Caspase-9 in apoptosis,thereby enhancing the sensitivity of NSCLC A549 to cisplatin.关键词
非小细胞肺癌/淫羊藿素/信号通路/细胞凋亡Key words
Non small cell lung cancer/Icaritins/Signal pathway/Apoptosis分类
中医学引用本文复制引用
马丁丁,石鑫,左宏波,王志,蒋轶..淫羊藿素对非小细胞肺癌的抑制作用研究[J].现代医药卫生,2025,41(6):1307-1310,1314,5.基金项目
江西省卫生健康委员会科技计划项目(SKJP220226105). （SKJP220226105）
