西南林业大学学报2025，Vol.45Issue(5)：202-207,6.DOI:10.11929/j.swfu.202407030
云南澄广花基因组大小的测定和分析
Prediction and Analysis of Genome Size of Orophea yunnanensis
摘要
Abstract
Flow cytometry and K-mer survey methods were utilized to estimate the genome size of Orophea yunnanensis,and analyzed the genome and its characteristics,and the DNA of Zea mays and Solunum lycopersic-um was used as standards in this study.The results showed that the genome size of O.yunnanensis was estimated to be approximately 866.53 Mb or 781.82 Mb by flow cytometry;125.97 Gb original data was obtained through high-throughout sequencing,and approximately high-quality 123.33 Gb clean data was acquired after processing,and this illustrated the sequencing data was good;the genome size of O.yunnanensis was about 782.90 Mb(K-mer=19),the heterozygous rate was about 0.86％;when K-mer=21,the genome size was estimated to be 781.20 Mb,and the heterozygous rate was about 0.90％;these suggested the genome of O.yunnanensis was highly het-erozygotic genome;the top 3 species of Chieniodendron hainanense,Alphonsea hainanensis and Stelechocarpus burahol were found to be the same family as O.yunnanensis compared using NT database.关键词
云南澄广花/流式细胞术/survey分析/基因组Key words
Orophea yunnanensis/flow cytometry/survey analysis/genome分类
农业科技引用本文复制引用
原晓龙,杨跃仙,卯梅华,张传光,王毅..云南澄广花基因组大小的测定和分析[J].西南林业大学学报,2025,45(5):202-207,6.基金项目
云南省"兴滇英才支持计划"教学名师项目(YNWR-JXMS-2019-038)资助 （YNWR-JXMS-2019-038）
云南省林业和草原科学院重点实验室开放基金项目(KFJJ21-03、KFJJ22-01)资助 （KFJJ21-03、KFJJ22-01）
云南省中青年学术和技术带头人后备人才项目(202205AC160044)资助. （202205AC160044）
