纤维素科学与技术2025，Vol.33Issue(2)：50-58,9.DOI:10.16561/j.cnki.xws.2025.02.05
动态转鼓发酵构建双层细菌纤维素基水凝胶及其疝修补潜力
Construction of Bilayer Bacterial Cellulose Hydrogel by Dynamic Drum Fermentation and Its Hernia Repair Potential
陈威 1陈琳 1洪枫1
作者信息
- 1. 东华大学 生物与医学工程学院,上海 201620
- 折叠
摘要
Abstract
The bilayer bacterial cellulose(BC)hydrogel with asymmetric pore structures on both sides(designated as BC/PS according to varying concentrations of potato starch)was successfully fabricated through dynamic fermentation using a horizontal rotating drum bioreactor,combined with a culture medium replacement strategy implementing gradient concentrations of gelatinized potato starch(PS)during the late fermentation stage.Systematic characterizations were conducted on its physicochemical properties and cytocompatibility.Analytical results revealed significant differences in the microporous architectures between the two surfaces:the starch-incorporated side developed a micron-scale porous structure(porous layer),leading to a remarkable enhancement in overall material porosity.With increasing PS concentrations,the density and tensile mechanical properties of BC/PS exhibited gradual decreases,while surface hydrophilicity and water retention capacity were markedly enhanced.Distinct protein adsorption capacities were observed between the two surfaces,with the dense layer demonstrating the lowest BCA protein adhesion rate(25.25%).In vitro cellular experiments confirmed that,compared to pristine BC,the porous layer structure of BC/PS significantly enhanced the adhesion and proliferation of L929 murine fibroblasts.These findings indicate that the dense BC layer possesses potential for preventing visceral adhesion,while the porous layer facilitates L929 cell proliferation.Such dual-functional characteristics endow BC/PS with promising application prospects in biomedical fields,particularly as hernia repair materials.关键词
细菌纤维素/动态转鼓发酵/糊化淀粉/原位培养改性/不对称孔隙结构/疝修补材料Key words
bacterial cellulose/dynamic drum fermentation/pregelatinized starch/in-situ culture modification/asymmetric pore structure/hernia repair material分类
基础医学引用本文复制引用
陈威,陈琳,洪枫..动态转鼓发酵构建双层细菌纤维素基水凝胶及其疝修补潜力[J].纤维素科学与技术,2025,33(2):50-58,9.
