新医学2025，Vol.56Issue(6)：560-567,8.DOI:10.12464/j.issn.0253-9802.2024-0362
杜仲叶和栀子提取物通过抑制自噬促进白色脂肪细胞褐变
Eucommia ulmoides leaves and Gardenia extracts promote white adipocyte browning by inhibiting autophagy
摘要
Abstract
Objective To investigate whether extracts of Eucommia ulmoides leaves and Gardenia have the effects on promoting white adipose browning,and to explore their possible mechanisms.Methods The effects of Eucommia ulmoides leaves and Gardenia extracts on mature 3T3-L1 cells were detected using CCK8 assay,Western blotting,qRT-PCR,Oil red O staining,BODIPY staining,and mitochondrial fluorescent dye.Subsequently,autophagy inhibitors(rapamycin)were used to explore their possible mechanisms of action.Results Eucommia ulmoides leaves and Gardenia extracts could upregulate browning-related proteins and mRNAs(PRDM16,PGC-1α and UCP-1)(all P<0.05),inhibited autophagy-related proteins(Beclin-1 and p62)and mRNAs(LC3,Beclin-1 and p62)and the expression of autophagosomes(all P<0.05),inhibited lipid droplet accumulation,and increased mitochondrial abundance.The use of autophagy activator(rapamycin)restored the autophagy effect of Eucommia ulmoides leaf and Gardenia extracts on 3T3-L1 cells(all P<0.05),and it was found that the browning effect of Eucommia ulmoides leaf and Gardenia extracts on 3T3-L1 cells was also reversed by autophagy activator(all P<0.05).Conclusion Extracts of Eucommia ulmoides leaves and Gardenia have the effects upon promoting the browning of white adipocytes by inhibiting autophagy,demonstrating potential clinical applications in the clinical prevention and treatment of obesity and related metabolic diseases.关键词
杜仲叶提取物/栀子提取物/自噬/白色脂肪细胞褐变/肥胖/脂滴积累Key words
Eucommia ulmoides leaf extract/Gardenia extract/Autophagy/Browning of white adipocyte/Obesity/Lipid droplet accumulation引用本文复制引用
熊绍风,胡智,方洋,赵乐,张洁,郭穆颖,魏莉,王昆..杜仲叶和栀子提取物通过抑制自噬促进白色脂肪细胞褐变[J].新医学,2025,56(6):560-567,8.基金项目
江西省中医药管理局科技计划重点项目(2020Z016) （2020Z016）
2024年江西省医院药学专项研究基金资助项目(2024-ZXYJ01) （2024-ZXYJ01）
