新医学2025，Vol.56Issue(6)：568-574,7.DOI:10.12464/j.issn.0253-9802.2024-0448
高蛋白低碳水化合物饮食对C57BL/6小鼠血脂和肠道短链脂肪酸分泌影响的研究
Effect of high-protein and low-carbohydrate diet on lipid and intestinal short-chain fatty acid secretion in C57BL/6 mice
摘要
Abstract
Objective To investigate and formulate the healthy high-protein and low-carbohydrate diet pattern suitable for the normal weight group.Methods Twenty-four SPF grade male C57BL/6 mice aged 6 to 8 weeks(weighing 15 to 20 g)were randomly divided into the control group(Ct group),5%high-protein low-carbohydrate diet group(5%LCD group),and 30%high-protein low-carbohydrate diet group(30%LCD group),with 8 mice in each group.The mice were fed with a basic diet,5%LCD,and 30%LCD for 4 weeks,respectively.The food intake,water intake,and body weight were observed,and the blood lipid indexes and the content of cecal short-chain fatty acids were measured.Results Compared with the Ct group,food intake in the 5%LCD and 30%LCD groups was increased(both P<0.01).Compared with the Ct group,the serum total cholesterol(TC)and low-density lipoprotein cholesterol(LDL-C)contents in the 5%LCD group were increased(both P<0.05),and the serum LDL-C and high-density lipoprotein cholesterol(HDL-C)levels in the 30%LCD group were increased(both P<0.05).Compared with the Ct group,the secretions of propionic acid,isobutyric acid,and isovaleric acid in the cecal contents of mice in the 5%LCD and 30%LCD groups were decreased(all P<0.05).Conclusion Based on a comprehensive assessment of body weight,lipid metabolism,and short-chain fatty acid secretion,30%LCD is more suitable as an effective dietary recommendation due to its higher HDL-C levels.关键词
高蛋白低碳水化合物/血脂/短链脂肪酸Key words
High-protein low-carbohydrate/Lipid/Short-chain fatty acid引用本文复制引用
贺语希,刘若楠,龚凯,胡拉来·依玛那力,李莉,马依拉·买买提,赵效国..高蛋白低碳水化合物饮食对C57BL/6小鼠血脂和肠道短链脂肪酸分泌影响的研究[J].新医学,2025,56(6):568-574,7.基金项目
国家自然科学基金(82160610) （82160610）
新疆维吾尔自治区自然科学基金(2022D01C444) （2022D01C444）
浙江诺特健康科技股份有限公司横向课题(NTKY-FP2023-001) （NTKY-FP2023-001）
