中国病理生理杂志2025，Vol.41Issue(6)：1066-1076,11.DOI:10.3969/j.issn.1000-4718.2025.06.003
RNA结合蛋白AUF1调控代谢相关基因表达及其在肝癌中的潜在作用
RNA-binding protein AUF1 regulates expression of metabolism-related genes and plays a potential role in hepatocellular carcinoma
摘要
Abstract
AIM:To investigate the expression of AU-rich element RNA-binding factor 1(AUF1),an RNA-binding protein,in hepatocellular carcinoma(HCC),and to explore its potential role in HCC progression through regula-tion of metabolism-related genes.METHODS:A tissue microarray containing 99 HCC samples and 95 adjacent nontu-morous liver tissues was used to assess AUF1 expression.The associations between AUF1 expression and HCC clinical pa-rameters were analysed using the GEPIA and UALCAN databases.The AUF1 gene was knocked down in human hepato-blastoma HepG2 cells by siRNA,and transcriptomic and TMT quantitative proteomic analyses were performed to identify alterations in metabolism-related genes.RESULTS:The AUF1 expression was significantly elevated in HCC tissues and correlated with a poor prognosis.Knockdown of AUF1 in HepG2 cells resulted in reduced cell viability and increased apop-tosis.Integrative analyses of transcriptomic and proteomic data revealed that AUF1 knockdown in HepG2 cells led to up-regulation of carboxylesterase 3(CES3),fibrinogen gamma chain(FGG)and 4-hydroxy-2-oxoglutarate aldolase 1(HOGA1),and down-regulation of lamin B receptor(LBR),riboflavin kinase(RFK),sterol O-acyltransferase 1(SOAT1)and TWIST neighbor(TWISTNB).Clinical data from GEPIA and UALCAN databases suggested that the expression of these metabolism-related genes in HCC patients exhibited an opposite trend.CONCLUSION:Our findings suggest that AUF1 is highly expressed in HCC,and may contribute to tumor progression and poor prognosis by modulating the expres-sion of a series of metabolism-related genes.关键词
AU富集元件RNA结合因子1/肝细胞癌/HepG2细胞/代谢调控/转录物组/蛋白质组Key words
AU-rich element RNA-binding factor 1/hepatocellular carcinoma/HepG2 cells/metabolic regu-lation/transcriptome/proteome分类
基础医学引用本文复制引用
吴莎莎,黄顺梅,韩群花,贾梦源,罗岁岁,朱丽君..RNA结合蛋白AUF1调控代谢相关基因表达及其在肝癌中的潜在作用[J].中国病理生理杂志,2025,41(6):1066-1076,11.基金项目
国家自然科学基金资助项目(No.82271587 （）
No.82200665) （）
