中国农业大学学报2025，Vol.30Issue(7)：184-195,12.DOI:10.11841/j.issn.1007-4333.2025.07.16
基于LC-MS/MS技术土壤中14种除草剂残留的检测方法
Detection method for 14 herbicide residues in soil based on LC-MS/MS technology
摘要
Abstract
The Modern Agricultural Research and Development Base in Pingdingshan City,Henan Province,was selected as the research object.A rapid method for the determination of bendazone,2-methyl-4-chlorophenoxyacetic acid,flusulfenoxuron and a total of 11 sulfonylurea herbicides in soil was established.Liquid Chromatography-tandem Mass Spectrometry(LC-MS/MS)technology was adopted.Samples were cleaned-up by stepwise extraction coupled with C18 solid-phase extraction,and quantified by an ACQUITY UPLC® HSS T3 column using a 1％formic acid aqueous solution-acetonitrile as mobile phase gradient.Combined with electrospray positive/negative ion switching multiple reaction monitoring mode,samples were quantitatively analyzed by using LC-MS/MS.The results showed that:1)A good linear relationship was observed between the injection mass fraction and the corresponding peak areas for the 14 herbicides at mass fractions ranging from 0.05 to 10 μg/kg,with the correlation coefficient of r≥0.99.The spiked recoveries ranged from 88.79％to 119.48％;2)The residues of acetamiprid in the inter-root soils of healthy and unhealthy leeks in non-healthy growing soil were higher than that in healthy leek inter-root soil,suggesting that ethofumesate residues might be potentially associated with unhealthy leek growth.In conclusion,this study provides technical support for the application of domestic LC-MS/MS instruments,and technical reference for the traceability of herbicides in farmland and the safety assessment of agricultural products.关键词
LC-MS/MS/除草剂残留/土壤/检测技术Key words
LC-MS/MS/herbicide residues/soil/detection technology分类
农业科技引用本文复制引用
常雪淼,李劲松,王宇欣,张明,王召,孙楠,陈建华,胡彬,马金海,马栋豪..基于LC-MS/MS技术土壤中14种除草剂残留的检测方法[J].中国农业大学学报,2025,30(7):184-195,12.基金项目
平顶山市重大科技专项(2020ZD19) （2020ZD19）
现代农业产业技术研究体系专项(CARS-23-C02) （CARS-23-C02）
