中国农学通报2025，Vol.41Issue(16)：14-20,7.
贝莱斯芽孢杆菌GDND-2对烟草的促生作用
Growth-promoting Effect of Bacillus velenzensis GDND-2 on Tobacco
摘要
Abstract
Bacillus velenzensis GDND-2 could effectively control tobacco Fusarium root rot.In order to elucidate its growth-promoting effects on tobacco,specific primers were used to amplify relevant growth-promoting genes.The capabilities for indole-3-acetic acid(IAA)production,siderophore secretion,and ACC deaminase activity were tested by Sackowski colorimetry,CAS agar plate assays,and sole nitrogen source methods respectively.The phosphorus and potassium solubilization capacities were detected by transparent circle method.Defense enzyme activities in tobacco after GDND-2 treatment were measured by assay kits.The growth-promoting effects of GDND-2 on tobacco were verified by potting experiment.Results revealed that GDND-2 possessed the growth-promoting gene ysnE and exhibited strong IAA production capacity,reaching 24.16 mg/L after fermentation for 36 h.The strain demonstrated solubilization of organic phosphorus,inorganic phosphorus,and potassium.GDND-2 fermentation broth significantly enhanced peroxidase(POD)activity in tobacco leaves,showing a 707.88%increase in POD activity after 96 h of treatment.The strain displayed remarkable growth-promoting effect on tobacco seedlings,increasing fresh weight by over 87.21%and root length by more than 75.61%.So Bacillus velezensis GDND-2 demonstrated excellent IAA secretion capacity and significantly promoted tobacco seedling growth,establishing itself as a high-quality biocontrol strain with superior characteristics.关键词
生防菌/贝莱斯芽孢杆菌GDND-2/促生基因/促生特性/吲哚乙酸/烟草/防御酶/促生效果Key words
biocontrol bacteria/Bacillus velenzensis GDND-2/growth-promoting genes/growth-promoting characteristics/IAA/tobacco/defense enzymes/growth-promoting effect分类
农业科技引用本文复制引用
沈会芳,杨祁云,张景欣,蒲小明,孙大元,刘平平,林壁润..贝莱斯芽孢杆菌GDND-2对烟草的促生作用[J].中国农学通报,2025,41(16):14-20,7.基金项目
广东省烟草科技计划项目"浈江流域上游主要作物病虫害绿色防控研究与应用"(201908) （201908）
广东省烟草科技计划项目"全生育期贯通的烟草绿色防控技术研发与推广"(202201). （202201）
