中国农学通报2025，Vol.41Issue(16)：21-27,7.
小麦黄色素相关分子标记的有效性验证
Validation of Molecular Markers Related to Yellow Pigment Content of Wheat Flour
摘要
Abstract
To advance molecular marker-assisted breeding of high yellow pigment(YP)wheat,this study validated the effectiveness of molecular markers associated with key enzymes(PSY,PDS,ZDS,and LCYE)in the YP biosynthesis pathway and oxidative enzymes(LOX,POD)responsible for flour bleaching.This was achieved by measuring flour YP content in 46 wheat varieties(lines).Functional markers YP7A and YP7B2 at the Psy-A1 and Psy-B1 loci,as well as dominant complementary functional markers LOX16 and LOX18 at the Lox-B1 locus,detected distinct allelic variations across the 46 wheat varieties(lines).The differences in mean flour YP content between these allelic variants reached highly significant levels(P<0.01).However,codominant functional markers YP4B1 and YP4B2 at the Pds-B1 locus showed no significant correlation(P=0.063)between allelic variations and flour YP content.Similarly,codominant functional markers POD3A1 and POD3A2 at the Pod-A1 locus revealed no significant differences in YP content between allelic variants,though Pod-A1a materials(low POD activity)exhibited slightly higher mean YP content compared to Pod-A1b materials(high POD activity).Functional markers YP7A,YP7B2,LOX16,and LOX18 are recommended for molecular marker-assisted breeding of high-yellow pigment wheat,whereas codominant markers YP4B1,YP4B2,POD3A1,and POD3A2 should be applied cautiously in such breeding programs.关键词
小麦/黄色素含量/黄色素相关基因/分子标记/等位变异/分子标记辅助育种Key words
wheat/yellow-pigment content/yellow-pigment related gene/molecular marker/allelic variation/marker-assisted breeding分类
农业科技引用本文复制引用
张楠,张士昌,彭义峰,李亚青,李孟军,史占良..小麦黄色素相关分子标记的有效性验证[J].中国农学通报,2025,41(16):21-27,7.基金项目
河北省小麦产业技术体系(HBCT2023010206) （HBCT2023010206）
石家庄市财政专项项目"高黄色素小麦种质创新与新品种选育"(2024-45). （2024-45）
