中国农学通报2025，Vol.41Issue(16)：45-50,6.
'航育1号'芦竹组培快繁体系的建立
An Efficient Micro Propagation System for Arundo donax'Hangyu No.1'
摘要
Abstract
In order to accelerate propagation of elite variety of Arundo donax'Hangyu No.1',and establish an efficient regeneration system,the stem segments of 3-year-old plants were used as explants to systematically explore the effects of sampling time and plant growth regulator combinations on the regeneration system.The results revealed that the best sampling time was in July and August,and the field plants were in a rapid growth period.The explant sterilization used 75%ethanol for 30 seconds and then 0.1%HgCl2 for 15-20 min,resulting in a survival rate of 90%approximately.The medium for axillary bud induction was MS+6-BA 4 mg/L+IBA 1 mg/L,92.66%buds developed obviously.Bud proliferation medium was MS+6-BA 7 mg/L+IBA 1 mg/L,resulting in a proliferation coefficient of 3.51.The rooting medium was 1/2MS+NAA 0.2 mg/L,and the rooting rate reached 97.36%.The best time for plant transfer to ex vitro conditions was 7-10 days after rooting.The survival rate was 92.22%in all the transferred plants.The plants were further moved to the field at the end of April and then under the standard field management.In November of the same year,the plant grew up to 3-3.5 m high with 30-50 tillers.This research provides solid technical support for large-scale production of'Hangyu No.1'.关键词
芦竹/'航育1号'/组织培养/快繁体系/茎段/生长调节剂/规模化生产Key words
Arundo donax/'Hangyu No.1'/tissue culture/efficient propagation system/stem/plant growth regulator/large-scale production分类
农业科技引用本文复制引用
李珊珊,刘宝平,李亚平,李晶炤,晏武,刘次次,王艺..'航育1号'芦竹组培快繁体系的建立[J].中国农学通报,2025,41(16):45-50,6.基金项目
北京市林草良种宣传示范推广项目"'航草B101'芦竹优良草品种宣传示范推广"(2024) （2024）
北京市科学技术协会金桥工程种子资金项目"芦竹多态性分子标记的开发与应用"(2024). （2024）
