中国农业大学学报2025，Vol.30Issue(8)：38-49,12.DOI:10.11841/j.issn.1007-4333.2025.08.04
拮抗灰葡萄孢木霉菌的鉴定及其防病作用
Identification of Trichoderma antagonizing Botrytis cinerea and its disease prevention effect
摘要
Abstract
The aims of this study were to identify the Trichoderma strains with antagonistic action against Botrytis cinerea from healthy grape berries and explore the key compounds that exert inhibitory effects and the control effect on grape gray mold By plate confrontation and buckle method,the target strain was identified by morphological observation and multi-gene phylogenetic analysis.The impact of volatile organic compounds(VOCs)emitted by Trichoderma spp.on the morphological alterations of B.cinerea mycelium was examined using scanning electron microscopy(SEM)and transmission electron microscopy(TEM).The components of Trichoderma VOCs were analyzed by gas chromatography-mass spectrometry(GC-MS).Finally,the antibacterial activity evaluation,semi-inhibitory concentration determination,and control effect test on grape gray mold of the identified VOCs were carried out using the fumigation method.The results showed that:1)The target Trichoderma strain GUI12 screened in this study was identified as Trichoderma citrinoviride;2)Compared with the plate confrontation,the inhibition effect of GUI12 on B.cinerea was better in the buckle culture,and the inhibition rate could reach 89.63％.3)SEM and TEM observation results showed that the VOCs produced by GUI12 could cause the mycelium of B.cinerea to become thicker,wrinkled and collapsed,and blur the outline of organelles including nucleus,mitochondria and vacuoles,even dissolve and disappear.4)The result of the in vitro grape disease resistance test showed that VOCs could effectively inhibit the expansion of grapefruit lesions,and the inhibition rate of grape gray mold lesion expansion was 53.56％.5)GC-MS analysis showed that GUI12 produced 19 types VOCs,such as isobutyl acetate,2,3-butanedione,phenylethyl alcohol,etc.They could inhibit the mycelial growth of B.cinerea in different degrees at the concentration of 100 μL/L,and the inhibition rates of 2,3-butanedione and Phenylethyl alcohol were 100％.6)The control effect of 2,3-butanedione on grape gray mold was 100％at EC50(14.97 μL/L).Phenethyl alcohol also achieved a 100％control effect on grape gray mold at 2×EC50(95.38 μL/L).In summary,the VOCs produced by GUI12 can inhibit the growth of B.cinerea hyphae,cause organelle damage,and effectively inhibit the spread of grape gray mold lesions.Among them,2,3-butanedione and phenylethyl alcohol have better control effects on grape gray mold and have the potential for further research and development.关键词
柠檬绿木霉/灰葡萄孢/挥发性有机化合物/气相色谱-质谱联用法/生物防治Key words
Trichoderma citrinoviride/Botrytis cinerea/VOCs/GC-MS/biocontrol resources分类
农业科技引用本文复制引用
石精涛,徐康文,曹建敏,丁应福,韩小斌,刘建阳,李喆,赵栋霖,张成省,林伟..拮抗灰葡萄孢木霉菌的鉴定及其防病作用[J].中国农业大学学报,2025,30(8):38-49,12.基金项目
国家自然科学基金项目(32302448) （32302448）
山东省自然科学基金项目(ZR2024QC002) （ZR2024QC002）
中央级公益性科研院所基本科研业务费专项(1610232023025) （1610232023025）
烟草常用生物农药新型增效剂的研究及应用(NYK2025-02-03) （NYK2025-02-03）
中国烟草总公司重大科技项目(110202101057(LS-17)) （110202101057(LS-17）
