中国农业大学学报2025，Vol.30Issue(8)：120-131,12.DOI:10.11841/j.issn.1007-4333.2025.08.11
鸡肌肉发育相关miR-133c-3p靶基因筛选与鉴定
Screening and identification of miR-133c-3p target genes related to chicken muscle development
摘要
Abstract
The aim is to investigate the expression of miR-133c-3p in different tissues and satellite cells of chickens,and to screen key target genes affecting muscle growth and development in pectoralis through bioinformatics analysis.In this study,an online website to predict chicken miR-133c-3p target genes was used,GO and KEGG enrichment analysis were performed and protein-protein interactions networks was constructed.Using an online public database,the expression of miR-133c-3p in pectoral muscle relative to other tissues was constructed and potential target genes were screened,and target validation by a dual luciferase reporter gene assay was performed.The results showed that:1)103 target genes were predicted;Functional enrichment analysis shows that target genes are involved in the synthesis and transport of various polymer compounds;And significantly enriched in multiple signaling pathways related to muscle growth and development,such as actin cytoskeleton regulation,mTOR,etc.2)Five Hub genes were identified through PPI network screening.3)MiR-133c-3p is only expressed in pectoralis and heart,and its expression decreases during satellite cell proliferation and increases during differentiation(P＜0.01).4)Four target genes,SGK1,MYH9,KLF7 and NFAT5,were identified through screening.5)Dual luciferase reporter gene detection revealed a target relationship between miR-133c-3p and NFAT5.In summary,chicken miR-133c-3p can indirectly regulate muscle growth and development by targeting NFAT5 in the pectoral muscle.This study provides a reference and basis for analyzing the function and molecular mechanism of miR-133c-3p in chickens.关键词
鸡/miR-133c-3p/生物信息学/基因表达量/肌肉生长发育/靶标验证Key words
chicken/miR-133c-3p/bioinformatics/gene expression level/muscle growth and development/target validation分类
农业科技引用本文复制引用
朱师良,邱莫寒,张增荣,杜龙环,汪江先,熊柱翔,杨朝武,陈家磊,彭涵,熊霞,胡陈明,杨礼,宋小燕,夏波,余春林..鸡肌肉发育相关miR-133c-3p靶基因筛选与鉴定[J].中国农业大学学报,2025,30(8):120-131,12.基金项目
四川省苗子工程培育项目(MZGC20240106) （MZGC20240106）
国家肉鸡产业技术体系项目(CARS-41-G07) （CARS-41-G07）
国家现代农业产业技术体系四川肉鸡创新团队项目(SCCXTD-2024-24) （SCCXTD-2024-24）
四川省省级科研院所基本科研项目(SASA202503) （SASA202503）
