中国农学通报2025，Vol.41Issue(17)：54-61,8.
不同施肥模式对土壤微生物多样性及群落结构的影响
Effects of Different Fertilization Practices on Soil Microbial Diversity and Community Structure
摘要
Abstract
This study explored the structural variations and diversity of soil rhizosphere microbial communities in dryland loam walnut orchards,aiming to establish a theoretical basis for improving high-quality walnut production.The research was conducted at the walnut experimental orchard of the Pomology Institute,Shanxi Agricultural University,through long-term positioning experiments utilizing various fertilization regimes.High-throughput sequencing of the 16S rRNA gene,along with one-way ANOVA,was employed to assess the community structures of soil rhizosphere bacteria and fungi under different fertilization treatments,including CK(no fertilization with natural grass cover),MG(sheep manure with artificial grass cover),and M(sheep manure with natural grass cover).The results revealed that organic fertilizer application significantly enhanced soil nutrient content,with the combination of sheep manure and natural grass cover exhibiting the most substantial effect.Furthermore,the application of organic fertilizers significantly improved soil enzyme activity,particularly when sheep manure was combined with artificial grass cover,thereby effectively accelerating soil nutrient cycling.Moreover,the integration of organic fertilization with grass cover had been shown to enhance bacterial and fungal populations and community structures,thereby increasing the abundance of microorganisms involved in nitrogen and carbon cycles and facilitating nutrient cycling and transformation.In the context of dryland conditions in Shanxi,the promotion of organic fertilization and inter-row grass cover in loam walnut orchards could significantly improve the structure and diversity of soil microbial communities,enhance soil nutrient availability,and optimize soil ecological conditions.关键词
不同施肥模式/根际土壤/微生物/生物群落及多样性/核桃园Key words
different fertilization patterns/rhizosphere soil/microorganisms/biological communities and diversity/walnut orchard分类
农业科技引用本文复制引用
王琦,史高川,田鑫,李阳,李夏媛,武艳霞,高鹏..不同施肥模式对土壤微生物多样性及群落结构的影响[J].中国农学通报,2025,41(17):54-61,8.基金项目
山西省青年科学研究项目"黄土高原区不同生草覆盖对土壤有机氮形态特征影响的微生物机制"(202203021212445) （202203021212445）
山西农业大学人才引进项目"旱作培肥对果园土壤有机态氮素转化的作用机制"(2022BQ15). （2022BQ15）
