中国病理生理杂志2025，Vol.41Issue(6)：1190-1199,10.DOI:10.3969/j.issn.1000-4718.2025.06.016
粪菌移植对C57BL/6J小鼠糖代谢及肠道菌群结构和短链脂肪酸代谢的影响
Effect of fecal microbiota transplantation on blood glucose metabolism and gut microbiota structure and short-chain fatty acid metabolism in C57BL/6J mice
摘要
Abstract
AIM:C57BL/6J mice were transplanted with fecal microbiota from a patient with maturity-onset diabetes of the young(MODY)or a healthy control.This study aimed to investigate the effects of fecal microbiota trans-plantation(FMT)on glucose and lipid metabolism,intestinal flora composition,and short-chain fatty acids(SCFAs)me-tabolism in mice,thereby providing an experimental foundation for understanding the role of gut microbiota in MODY.METHODS:A total of 36 male C57BL/6 mice were randomly allocated into three groups,including:control(CON)group(receiving PBS),normal individual-FMT(NF)group(intervened with normal human fecal microbiota)and MODY patient-FMT(MF)group(intervened with MODY patient fecal microbiota),and the mice were performed fecal microbio-ta,transplantation through oral gavage for 8 weeks.Glucose tolerance was assessed using an oral glucose tolerance test(OGTT),while insulin sensitivity was evaluated through an intraperitoneal insulin tolerance test(ITT).Blood biochemi-cal indicators were measured using ELISA,the composition of the gut microbiota was analyzed using 16S rRNA sequenc-ing,and the concentration of fecal short-chain fatty acids(SCFAs)was determined by gas chromatography-mass spec-trometry(GC-MS).RESULTS:The mice in MF group showed impaired glucose tolerance and reduced insulin sensitivi-ty.The FMT altered the composition of gut microbiota in mice,leading to significant differences in microbial diversity among groups.Specifically,the distribution of 18 bacterial species varied significantly among the three groups,with a no-table reduction in SCFA-producing bacteria observed in the MF group.Analyses of fecal SCFAs revealed that acetic acid levels were significantly lower in the MF group(P<0.05).CONCLUSION:The FMT reduces production of SCFAs by altering gut microbiota composition in mice,which was likely related to impaired glucose tolerance and decreased insulin sensitivity.关键词
青少年起病的成人型糖尿病/肠道菌群/粪菌移植/16S rRNA/短链脂肪酸Key words
maturity-onset diabetes of the young/gut microbiota/fecal microbiota transplantation/16S rRNA/short-chain fatty acids分类
医药卫生引用本文复制引用
罗倩倩,庞越胜,徐逸凡,蒋靓,帕它木·莫合买提..粪菌移植对C57BL/6J小鼠糖代谢及肠道菌群结构和短链脂肪酸代谢的影响[J].中国病理生理杂志,2025,41(6):1190-1199,10.基金项目
新疆维吾尔自治区科技厅天山雪松计划(No.2019XS03) （No.2019XS03）
新疆维吾尔自治区"十四五"高等学校特色学科-公共卫生与预防医学 （）
