中国蔬菜Issue(6)：74-84,11.DOI:10.19928/j.cnki.1000-6346.2025.0040
基于加权基因共表达网络挖掘番茄抗灰霉病的关键基因
Identification of Hub Genes in Tomato Resistance to Gray Mold Based on Weighted Gene Co-expression Network Analysis
摘要
Abstract
To identify hub genes involved in tomato resistance to gray mold,transcriptome sequencing was performed on leaves of tomato high-generation inbred line'E6203'seedlings before and after infection.Weighted gene co-expression network analysis(WGCNA)was employed to screen for hub genes associated with tomato resistance to Botrytis cinerea.The results showed that WGCNA identified 13 co-expression gene modules,of which two modules were associated with the early response and two modules were associated with the late response to B.cinerea infection in tomato.Functional enrichment analysis revealed that primary metabolic processes were downregulated after B.cinerea infection,while defense response processes were activated.Further analysis of hub genes within these modules led to the identification of 4 candidate genes,including CDIP,DJC17,NATA2,and NPF6.4,that may be involved in regulating resistance to gray mold.The defense response to B.cinerea in tomato mainly involves pathways such as glycogen catabolic,pyridine-containing compound catabolic,and membrane protein proteolysis.关键词
番茄/灰霉病/转录组测序/加权基因共表达网络分析/关键基因Key words
tomato/gray mold/transcriptome sequencing/weighted gene co-expression network analysis(WGCNA)/hub gene引用本文复制引用
张孟晗,韩鸿宇,李亚妮,耿梦爽,刘奕飞,刘佳琳,孟宪文,李传友..基于加权基因共表达网络挖掘番茄抗灰霉病的关键基因[J].中国蔬菜,2025,(6):74-84,11.基金项目
青岛市科技惠民示范工程项目(23-2-8-xdny-15-nsh),国家重点研发计划项目(2023YFD1200100),国家自然科学基金项目(32000364) （23-2-8-xdny-15-nsh）
