中国草地学报2025，Vol.47Issue(6)：21-33,13.DOI:10.16742/j.zgcdxb.20250019
温性典型草原优势植物叶际微生物群落对放牧的响应
Responses of Phyllosphere Microbial Community of Dominant Plant to Grazing in Temperate Typical Steppe
摘要
Abstract
The response of grassland plants to grazing pressure and their subsequent growth dynamics are critical for maintaining ecosystem stability.As the primary interface for grazing interactions,leaf growth status is not only regulated by external environmental factors,but also significantly influenced by the metabolic activities of epiphytic microorganisms.This research investigated the responses of epiphytic microbial community characteristics of dominate species to grazing in temperate typical grasslands of north-ern China.High-throughput sequencing technology was applied to analyze the diversity and community composition of phyllosphere bacterial and fungal of Leymus chinensis,Stipa grandis,and Cleistogenes squarrosa.Results showed that:(1)Under both grazing and enclosure conditions,the epiphytic microbial communities of all three plant species were consistently dominated by bacterial phyla including Pseudomo-nadota,Bacillota,Bacteroidota,Actinobacteriota,and Acidobacteriota,along with fungal phyla Ascomy-cota and Basidiomycota.Notably,grazing disturbance significantly reduced the relative abundance of Pro-teobacteria in L.chinensis phyllosphere communities,creating ecological niches for colonization of other microbial taxa.Under grazing conditions,concurrently,a significant reduced in Filobasidium abundance within fungal communities of L.chinensis was observed,This change may reduce the risk of plant disease.(2)Under grazing conditions,the phyllosphere exhibited an enrichment of unique microbial taxa.Thermo-togota was detected for the first time in bacterial communities of L.chinensis,while Kickxellomycota was identified as novel colonizer in fungal communities of C.squarrosa.These newly identified microbial taxa may significantly influence the adaptability and functional diversity of phyllosphere microenvironments.(3)The responses of functional classification in phyllosphere fungi to grazing and host specificity were signifi-cantly stronger.Under grazing conditions,relative abundance of pathogenic fungal decreased,whereas ben-eficial fungi increased,indicating that grazing may indirectly suppress pathogen proliferation by altering the phyllosphere microenvironment.In conclusion,effects of grazing directly alters phyllosphere microbial community structure by modifying host plant characteristics and their associated microenvironments,ulti-mately increasing microbial diversity and the proportion of beneficial microorganisms.关键词
典型草原/放牧/叶际/微生物/功能类群Key words
Typical steppe/Grazing/Phyllosphere/Microorganism/Functional taxa分类
农业科技引用本文复制引用
王彤,李西良,唐士明,王忠武..温性典型草原优势植物叶际微生物群落对放牧的响应[J].中国草地学报,2025,47(6):21-33,13.基金项目
国家自然科学基金项目(32271649,U23A2001,32460-353) （32271649,U23A2001,32460-353）
中国农业科学院青年创新人才专项(Y2022QC29) （Y2022QC29）
一流学科科研专项(YLXKZX-NND-002) （YLXKZX-NND-002）
