中国蔬菜Issue(6)：129-135,7.DOI:10.19928/j.cnki.1000-6346.2025.5021
河北保定番茄黄化曲叶病毒病及棚外杂草寄主鉴定
Molecular Identification of Tomato Yellow Leaf Curl Virus Disease and Weeds Outside Greenhouse in Baoding,Hebei Province
摘要
Abstract
In response to the incidence of viral diseases in facility tomatoes and weeds outside greenhouses in Baoding,Hebei Province,leaf samples of facility tomatoes,humus grass,and quinoa with twisted deformities,mottled,elongated and narrow,protruding,and longitudinally rolled leaves were collected separately.Use tomato virus disease specific primers for RT-PCR amplification and identification of total RNA in tomato,Humulus scandens,and Chenopodium album.The results showed that the specific primer TYLCV amplified a single band of approximately 648 bp on tomato,Humulus scandens,and Chenopodium album,while other primers and negative controls did not amplify.This study confirms that the Tomato yellow leaf curl virus(TYLCV)is present in facility tomatoes and weeds outside the greenhouse,indicating that weeds(such as Humulus scandens and Chenopodium album)around facility tomatoes are suspected hosts of TYLCV and have become one of the sources of viral infection.The results of this study can provide technical support for early detection and comprehensive prevention and control of tomato viral diseases in facilities.关键词
番茄/葎草/藜/番茄黄化曲叶病毒/寄主Key words
tomato/Humulus scandens/Chenopodium album/Tomato yellow leaf curl virus(TYLCV)/host引用本文复制引用
王云端,高兴,张胜杰,宫彬彬,吴晓蕾,吕桂云,李敬蕊,陈利达,高洪波..河北保定番茄黄化曲叶病毒病及棚外杂草寄主鉴定[J].中国蔬菜,2025,(6):129-135,7.基金项目
河北农业大学引进人才科研专项(YJ2024011),河北省自然科学基金项目(C2024204182),河北省现代农业产业技术设施蔬菜创新团队项目(HBCT2023100211) （YJ2024011）
