中国人兽共患病学报2025，Vol.41Issue(5)：501-507,7.DOI:10.3969/j.issn.1002-2694.2025.00.069
我国不同生态环境白蛉的遗传进化特征及其对疾病传播的影响
Genetic evolution characteristics and their influence on disease transmission in sandflies in various environments in China
摘要
Abstract
This study analyzed the genetic evolutionary characteristics of sandflies and their effects on the spread of kala-azar in various environments in endemic provinces in China,to provide a scientific basis for kala-azar disease prevention and control.Sand-flies were collected in kala-azar endemic areas such as southern Xinjiang,the large hilly areas of southern Gansu,the northern Sich-uan and Taihang Mountains,and surrounding small hills.The cytochrome c oxidase subunit I and cytochrome b gene fragments of mito-chondrial DNA were amplified to identify sandfly species.The COI and Cytb gene sequences of sandflies from southern Xinjiang and Si-chuan recorded in NCBI were also collected.The intraspecific and interspecific genetic differences of sandflies were calculated in MEGA11.0,and a phylogenetic tree was constructed through the neighbor-joining method,for analysis of the genetic and evolutionary characteristics of sandfly populations and their effects on disease transmission.A total of 155 sandflies were collected from nine sam-pling sites in seven provinces of China;the species included Phlebotomus chinensis,Phlebotomus wui,and Sergentomyia squamirostris.Five sandfly species belonging to two genera were collected:P.chinensis,P.wui,and Phlebotomus alexandri in the genus Phleboto-mus,and S.squamirostris in the genus Sergentomyia.Genetic evolution analysis based on COI and Cytb gene sequences indicated intra-specific genetic distances of 0-0.062 and 0-0.056,respectively,and interspecific genetic distances of 0.126-0.176 and 0.110-0.171,respectively.The phylogenetic tree indicated that P.wui,P.alexandri,Phlebotomus longiductus,and S.squamirostris clus-tered into one branch.The sequences of P.chinensis in the large and small hilly areas clustered into two geographical clades.In the small hilly areas,the sequences of P.chinensis aggregates showed small genetic differences,the pathogen infection was consistent,and the cases showed an epidemic spread trend.Large genetic differences at the molecular level were observed among sandflies in dif-ferent ecological regions,thus indicating key effects on leishmaniasis transmission.On the basis of these findings,prevention and con-trol strategies should be adapted to local conditions,and precise and effective prevention and control measures should be formulated according to the genetic evolution characteristics of sandflies in different regions,to better control the transmission of Kala-azar.关键词
白蛉/生态环境/黑热病/细胞色素c氧化酶亚基I/细胞色素b/遗传进化Key words
sandfly/ecological environments/Kala-azar/COI gene/Cytb gene/genetic evolution分类
基础医学引用本文复制引用
崔蕾,何雅琦,周正斌,方圆,李中秋,李元元,杨丽敏,张仪..我国不同生态环境白蛉的遗传进化特征及其对疾病传播的影响[J].中国人兽共患病学报,2025,41(5):501-507,7.基金项目
国家重点研发计划(No.2023YFA1801000,No.2023YFA1801001) National Key Research and Development Program of China(No.2023YFA1801000,No.2023YFA1801001) （No.2023YFA1801000,No.2023YFA1801001）
