中国草地学报2025，Vol.47Issue(6)：90-99,10.DOI:10.16742/j.zgcdxb.20240365
长刺蒺藜草生防菌TL-3分离鉴定及除草效果研究
Isolation,Identification and Weed Control Effect Study of Alternaria alternata Strain TL-3 from Cenchrus longispinus
摘要
Abstract
In this study,strain TL-3 was isolated from naturally infected Cenchrus longispinus,and identified as Alternaria alternata by morphological and molecular biology.The herbicidal activity of TL-3 was determined by paper dish method and indoor biocontrol test.The results showed that the inhibition rate of TL-3 fermentation broth on the growth of roots and buds of C.longispinus was 67.65%,59.65%,respectively.The incidence rate of stem and leaf diseases was as high as 63.89%.However,the inhibition rate of TL-3 on the germination of C.longispinus seeds was relatively weak,with an inhibition rate of only 10.02%.The herbicidal activity of TL-3 fermentation broth on six kinds of common farmland weeds showed that it had obvious pathogenic effect on Chenopodium album,Echinochloa crusgalli,Amaranthus retroflexus and Chloris virgata.The disease incidence was 85.65%,84.94%,50.91%,41.51%,and the disease index was 85.93,87.04,44.35 and 45.15,respectively.The incidence and disease index of C.album and E.crusgalli were significantly higher than those of other weeds(P<0.05).The disease incidence of Setaria viridis and Digitaria sanguinalis were 23.28%and 29.25%,respectively,and the dis-ease index was 29.44,and 27.98,respectively.The results showed that the disease incidence of Medi-cago sativa and Melilotus officinalis was 45.07%and 40.88%,respectively,and the disease index was 40.26 and 44.39,respectively.The disease incidence of M.sativa was significantly higher than that of other forage and crops(P<0.05),and the disease index of M.officinalis was significantly higher than that of other forage and crops(P<0.05).The incidence rates of maize,wheat,oats,and Medicago ruthenica were 26.32%,24.32%,29.07%,and 28.90%,respectively,and the incidence rates of Glycine max,Solanum lycopersicum,Brassica oleracea,and Brassica pekinensis were 11.11%,10.71%,14.92%,and 9.21%,respectively,Solanum melongena,Capsicum annuum,Elymus dahuricus,Leymus chinensis,Cucumis sativus and Spinacia oleracea showed no obvious symptoms compared with the control group.The results indicated that strain TL-3 was safe.In summary,strain TL-3 had a significant pathogenic effect on C.longispinus and can be safely used in artificial grasslands/or wastelands mainly composed of L.chinensis.It is a new solution for the control of C.longispinus and has the potential to be developed as a bioherbicide.关键词
长刺蒺藜草/互隔交链孢霉/杂草/安全性/生物防治Key words
Cenchrus longispinus/Alternaria alternata/Weed/Security/Biocontrol分类
农业科技引用本文复制引用
王桦,陈艺豪,徐晓阳,郭丽珠,李宇宇,苏宏华,郝丽芬,林克剑..长刺蒺藜草生防菌TL-3分离鉴定及除草效果研究[J].中国草地学报,2025,47(6):90-99,10.基金项目
国家重点研发计划项目(2023YFD1400505) （2023YFD1400505）
国家牧草产业技术体系(CARS-34) （CARS-34）
中央级公益性科研院所基本科研业务费专项(1610332023002) （1610332023002）
