中国人兽共患病学报2025，Vol.41Issue(5)：522-528,7.DOI:10.3969/j.issn.1002-2694.2025.00.072
人工繁育梅花鹿源大肠埃希菌分离鉴定及耐药性与毒力基因分析
Isolation,identification,and analysis of drug resistance and virulence genes in Escherichia coli isolated from artificially bred sika deer
摘要
Abstract
To understand the background of Escherichia coli(E.coli)carried by artificially bred sika deer and the biological characteristics of the isolated strains,such as drug resistance and pathogenicity,in April 2024,we collected 184 fresh deer fecal samples from four deer farms in Luxiang Township,Shuangyang District,Changchun City,Jilin Province,for isolation and cultivation of E.coli.The isolates were tested for drug resistance and biochemical identification with a BD PhoenixTM-100 Automated Microbiology System.The virulence genes were detected with PCR,and the strains were molecularly typed with ERIC-PCR.A total of 165 E.coli strains were isolated from 184 samples of deer feces,with an isolation rate of 89.67%.Twenty strains had a drug resistance phenotype,and the drug resistance rate was 12.12%;these strains included 15 strains of multi-drug resistant bacteria and 11 strains of ESBL-producing bacteria.Virulence gene detection indicated that the sika deer isolates carried multiple diarrhea-associated virulence genes,such as EAST-1(12.12%),eae(1.21%),stx1(7.88%),stx2(7.27%),and STa(1.82%).ERIC-PCR demonstrated that the isolates showed high polymorphism.The ESBL-producing E.coli carried by sika deer are likely to spread drug resistance in the community and livestock population.Some isolates carried multiple diarrhea-associated virulence genes,thus posing a human transmission risk.Therefore,monitoring of drug resistance and virulence genes must be strengthened,and antibiotics must be used reasonably during the breeding process to avoid excessive use and misuse.关键词
梅花鹿/大肠埃希菌/耐药性/毒力基因/ERIC-PCRKey words
sika deer/Escherichia coli/drug resistance/virulence gene/ERIC-PCR分类
基础医学引用本文复制引用
张成阳,纪雪,姜博文,梁冰,黄荣磊,杜崇涛,孙洋..人工繁育梅花鹿源大肠埃希菌分离鉴定及耐药性与毒力基因分析[J].中国人兽共患病学报,2025,41(5):522-528,7.基金项目
国家重点研发计划重点专项(No.2023YFF1305401) （No.2023YFF1305401）
国家林业和草原局野生动物疫病监测和预警项目(No.[2023]58) National Key Research and Development Project of China(No.2023YFF1305401) （No.[2023]58）
The Wildlife Epidemic Monitoring Project of National Forestry and Glassland Bureau of China([No.2023]58) （[No.2023]58）
