中国输血杂志2025，Vol.38Issue(6)：747-758,12.DOI:10.13303/j.cjbt.issn.1004-549x.2025.06.001
血小板对5种常见致病菌抗菌效果的比较分析
Antibacterial effects of platelets on five common pathogenic bacteria:a comparative analysis
摘要
Abstract
Objective To compare and analyze the antibacterial effects of platelets against five common clinical patho-genic bacteria including MRSA,SE,SA,E.coli,and CRKP,and to preliminarily explore the role of DCD sensitivity in the observed variations of antibacterial effects.Methods The same number of platelets were used to establish co-culture sys-tems of platelets and platelet lysates with the five pathogenic bacteria.The antibacterial effects of platelets and platelet lysat-es on the five pathogenic bacteria were evaluated by observing the turbidity of the bacterial solution,measuring the OD600 nm value of the bacterial solution and counting the colonies.The supernatant protein of platelets co-cultured with MRSA was collected for quantitative proteomics analysis to explore the important antibacterial proteins of platelets.The content of DCD in the supernatant after co-culture of platelets and platelet lysates with the five pathogenic bacteria was detected by ELISA to preliminarily analyze the reasons for the different antibacterial effects of platelets on the five pathogenic bacteria.Results Compared with the control group of MRSA,SA,and SE,the turbidity of the bacterial solution decreased after co-culture of platelets and platelet lysates with MRSA,SA,and SE for 12 h,and the OD600 nm value and colony count were significantly reduced(P<0.05).The turbidity of the bacterial solution did not change significantly after co-culture of platelets and plate-let lysates with E.coli for 24 h,but the OD600 nm value decreased(P<0.05),and the colony count decreased to 108 CFU/mL but the difference was not statistically significant(P>0.05).Compared with the control group of CRKP,the turbidity,OD600 nm value,and colony count of the bacterial solution did not change significantly after co-culture of platelets and platelet lysates with CRKP(P>0.05).Proteomics results showed that after co-culture with MRSA,important proteins related to platelet activation,including collagen,fibrinogen,von Willebrand factor,integrin αIIbβ3,platelet glycoprotein V and IV were significantly up-regulated.ELISA results showed that after co-culture with the five pathogenic bacteria,platelets could secrete a large amount of DCD,with the content around 3 μg/mL.Conclusion The antibacterial effect of platelets on Gram-positive bacteria MRSA,SA,and SE is better than that on Gram-negative bacteria E.coli and CRKP,and platelets have the best antibacterial effect on MRSA.The differences in antibacterial effects of platelets on the five pathogenic bacteri-a may be related to the sensitivity of DCD antibacterial peptides to the five pathogenic bacteria.关键词
血小板抗菌/血小板活化/皮离蛋白/耐甲氧西林金黄色葡萄球菌/革兰氏阳性菌/革兰氏阴性菌Key words
platelet antibacterial/platelet activation/dermcidin/MRSA/gram-positive bacteria/gram-negative bacte-ria分类
医药卫生引用本文复制引用
刘二雄,王文婷,徐金梅,安宁,王雅芬,时洁芸,张玲玲,陈要臻,尹文..血小板对5种常见致病菌抗菌效果的比较分析[J].中国输血杂志,2025,38(6):747-758,12.基金项目
陕西省自然科学基础研究计划(2024JC-YBQN-0890) （2024JC-YBQN-0890）
