中国输血杂志2025，Vol.38Issue(6)：759-765,7.DOI:10.13303/j.cjbt.issn.1004-549x.2025.06.002
储存条件对长期保存PRP生长因子的影响
Effect of storage conditions on long-term preservation of PRP growth factors
摘要
Abstract
Objective To compare the changes in the concentration of relevant growth factors released from platelet-rich plasma(PRP)stored at-80℃by cryopreservation and at 4℃by refrigerated lyophilization over 2 years,aiming to provide a theoretical basis for prolonging PRP storage duration.Methods PRP(n=15)was separated using a blood cell separator and stored under-80℃cryopreservation(F-PRP group)and 4℃refrigerated freeze-drying conditions(FD-PRP group).The contents of growth factors(PDGF-AA,PDGF-BB,EGF,TGF-β1,and VEGF)in both groups were measured by ELISA at 1,3,6,9,12 and 24 months.Results PDGF-AA and VEGF maintained good stability in both groups for up to 24 months.PDGF-BB and TGF-β1 showed high stability in the first 12 months but their stability decreased gradually from 12th to 24th months.EGF demonstrated good stability in the first 6 months,and its stability gradually decreased from the 9th to 24th months.Comparing the F-PRP and FD-PRP groups,the concentrations of the five growth factors in the FD-PRP group were either not statistically different or higher than those in the F-PRP group at all time points.Specifically,the concentra-tions of EGF were significantly higher in the FD-PRP group at all time points.Conclusion Both-80℃freezing and 4℃freeze-drying enable long-term preservation of PRP.Freeze-drying imposes less stringent storage requirements and facilitates growth factor compared to frozen storage.关键词
富血小板血浆/生长因子/储存时间/冰冻/冻干Key words
platelet-rich plasma/growth factor/storage time/frozen/freeze-dried分类
医药卫生引用本文复制引用
齐清,李兆杰,吴琼,毛平平,孙杨子,栾建凤,王淑君..储存条件对长期保存PRP生长因子的影响[J].中国输血杂志,2025,38(6):759-765,7.基金项目
东部战区总医院院内课题(22LCZLXJS52) （22LCZLXJS52）
