中国输血杂志2025，Vol.38Issue(6)：824-827,4.DOI:10.13303/j.cjbt.issn.1004-549x.2025.06.012
不同保存温度和时间对全血中凝血因子Ⅷ和凝血因子Ⅸ活性的影响研究
Effects of different storage temperatures and durations on the activity of coagulation factor Ⅷ and Ⅸ in whole blood
摘要
Abstract
Objective To investigate the effects of different storage temperatures and durations on the activities of coag-ulation factor Ⅷ(Factor Ⅷ,FⅧ)and coagulation factor Ⅸ(Factor Ⅸ,FⅨ)after whole blood collection,so as to pro-vide data support for the optimal storage conditions.Methods A total of 16 mL of whole blood was collected from each of the 20 healthy volunteers at our blood center and aliquoted into 8 sodium citrate anticoagulant tubes.Two tubes were imme-diately centrifuged for the measurement of FⅧ and FⅨ activity levels.The remaining 6 tubes of whole blood were respec-tively stored under room temperature and low-temperature conditions.At 2,4,and 6 h,the whole blood samples were cen-trifuged and analyzed for FⅧ and FⅨ activity levels.The mean values of the two immediately tested tubes were used as the control group,while other tubes were designated as the experimental groups for comparison.Statistical analysis was per-formed using SPSS 26.0.Results The activity of FⅧ in whole blood remained stable after 4 hours of storage at both room temperature and low temperature(116.53±25.95 vs 125.22±27.33,109.77±23.23 vs 125.22±27.33)(P>0.05 for both).However,by 6 hours,FⅧ activity showed a statistically significant decline compared to the control group(108.65±22.92 vs 125.22±27.33,100.46±20.19 vs 125.22±27.33)(P<0.05 for both),though the room temperature group re-sults were closer to the control values.The activity of FⅨ in whole blood remained stable after 6 hours of storage under both conditions(97.14±19.48 vs 96.76±19.67,97.10±17.45 vs 96.76±19.6)(P>0.05 for all comparisons).Conclusion For whole blood samples after collection,storage at either room temperature or low temperature for up to 4 hours does not compromise the accuracy of test results.When stored for 6 hours,FⅨ activity remains stable,whereas FⅧ activity decrea-ses significantly.Notably,FⅧ activity demonstrates better stability at room temperature than under low-temperature condi-tions within the 6-hour storage.关键词
凝血因子Ⅷ/凝血因子Ⅸ/保存温度/全血Key words
factor Ⅷ/factor Ⅸ/storage temperature/whole blood分类
医药卫生引用本文复制引用
王贺贺,王甜甜,王杰,乔翠翠,刘薇,张雪芹,程彦,房云海,张心声..不同保存温度和时间对全血中凝血因子Ⅷ和凝血因子Ⅸ活性的影响研究[J].中国输血杂志,2025,38(6):824-827,4.基金项目
山东省医药卫生科技项目(202314011476) （202314011476）
