中国畜牧兽医2025，Vol.52Issue(6)：2809-2817,9.DOI:10.16431/j.cnki.1671-7236.2025.06.033
CRISPR/Cas系统在寄生虫病诊断领域的研究进展
Research Progress on CRISPR/CAS System in the Diagnosis of Parasitic Diseases
摘要
Abstract
The detection of animal parasitic diseases has mainly relied on the combination of traditional microscopy and PCR technology for many years.It has indeed played a significant role in clinical practice,and has also controlled the prevalence of some parasitic diseases.However,parasitic diseases are mostly chronic consumptive diseases,and the significance of early diagnosis is particularly important.The sensitivity and simplicity of traditional methods cannot meet the needs of complete control of parasitic diseases.Therefore,the promotion of the use of more sensitive and more convenient detection methods are urgently needed.In recent years,the CRISPR/Cas system has been continuously developed,and a variety of Cas protein functions have been discovered and widely used in nucleic acid detection techniques for parasitic pathogens.The researchers screened the specific target sequence and designed crRNA for the combination of Cas13 protein and Cas12 proteins with recombinase polymerase amplification(RPA)or PCR in the type 2 CRISPR/Cas system,and then combined with Cas12,Cas13 proteins and fluorescent reporter group to form a CRISPR/Cas nucleic acid detection system,which can be used for the detection of various parasitic diseases.It has the characteristics of fast,accurate,convenient and cheap,and is suitable for on-site detection,and has great market application potential.In this paper,the mechanism and classification of CRISPR/Cas system and its research progress in the field of nucleic acid diagnosis of parasitic diseases in recent years are reviewed,so as to provide reference for the early diagnosis of parasitic diseases.关键词
CRISPR/Cas系统/Cas13/Cas12/crRNA/核酸检测Key words
CRISPR/Cas system/Cas13/Cas12/crRNA/nucleic acid detection分类
农业科技引用本文复制引用
刘浩,张昊,张艳敏,张丽丽,王文龙..CRISPR/Cas系统在寄生虫病诊断领域的研究进展[J].中国畜牧兽医,2025,52(6):2809-2817,9.基金项目
国家乳业技术创新中心项目(2023-JSGG-5) （2023-JSGG-5）
